› 6nmm

X-ray diffraction
2.5Å resolution

Ternary complex structure of the T130K mutant of ANT-4 with Neomycin, AMPCPP and Pyrophosphate

Released:
DOI: 10.2210/pdb6nmm/pdb
PDB entry 6nmm coloured by chain, front view.
PDB entry 6nmm coloured by chain, side view.
PDB entry 6nmm coloured by chain, top view.
Source organism: Geobacillus stearothermophilus
Primary publication:
'Catch and Release": a Variation of the Archetypal Nucleotidyl Transfer Reaction
Selvaraj B, Kocaman S, Trifas M, Serpersu EH, Cuneo MJ
ACS Catalysis (2020)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176427 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Kanamycin nucleotidyltransferase C-terminal domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 256 amino acids
Theoretical weight: 29.14 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Escherichia coli-Pichia pastoris shuttle vector pPpARG4
UniProt:
  • Canonical: Q57514 (Residues: 1-253; Coverage: 100%)
Sequence domains: KNTase C-terminal domain

Ligands and Environments

4 bound ligands:
Ligand NMY 6 x NMY
Ligand A 6 x A
Ligand PPV 4 x PPV
Ligand MG 8 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P21
Unit cell:
a: 85.51Å b: 59.25Å c: 102.04Å
α: 90° β: 94.8° γ: 90°
R-values:
R R work R free
0.176 0.174 0.23
Expression system: Escherichia coli-Pichia pastoris shuttle vector pPpARG4

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