› 6nl7

X-ray diffraction
1.4Å resolution

Crystal structure of B1 immunoglobulin-binding domain of Streptococcal Protein G (T16F, T18A, V21H, T25H, K28Y, V29I, K31R, Q32A, Y33L, N35K, D36A, N37Q)

Released:
DOI: 10.2210/pdb6nl7/pdb
PDB entry 6nl7 coloured by chain, front view.
PDB entry 6nl7 coloured by chain, side view.
PDB entry 6nl7 coloured by chain, top view.
Source organism: Streptococcus
Primary publication:
Design of High-Affinity Metal-Controlled Protein Dimers.
Maniaci B, Lipper CH, Anipindi DL, Erlandsen H, Cole JL, Stec B, Huxford T, Love JJ
Biochemistry 58 2199-2207 (2019)
PMID: 30938154

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148791 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Immunoglobulin G-binding protein G Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 56 amino acids
Theoretical weight: 6.25 KDa
Source organism: Streptococcus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P19909 (Residues: 303-357; Coverage: 10%)
Gene name: spg
Sequence domains: B domain
Structure domains: Ubiquitin-like (UB roll)

Ligands and Environments

6 bound ligands:
Ligand ZN 4 x ZN
Ligand ACT 8 x ACT
Ligand NA 4 x NA
Ligand CL 1 x CL
Ligand PO4 2 x PO4
Ligand DPO 1 x DPO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: P21
Unit cell:
a: 40.903Å b: 63.057Å c: 53.225Å
α: 90° β: 103.31° γ: 90°
R-values:
R R work R free
0.115 0.113 0.158
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 citations in other articles

Biofabrication of functional protein nanoparticles through simple His-tag engineering.
López-Laguna et al. (2021)