› 6nl6

X-ray diffraction
1.4Å resolution

Crystal structure of mutant B1 immunoglobulin-binding domain of Streptococcal Protein G (T16F, T18A, V21E, T25L, K28Y, V29I, K31R, Q32H, Y33L, N35K, D36H, N37Q)

Released:
DOI: 10.2210/pdb6nl6/pdb
PDB 6nl6 coloured by chain and viewed from the front.
PDB 6nl6 coloured by chain and viewed from the side.
PDB 6nl6 coloured by chain and viewed from the top.
Source organism: Streptococcus
Entry authors: Huxford T, Boguslaw S, Maniaci B

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Immunoglobulin G-binding protein G Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 56 amino acids
Theoretical weight: 6.35 KDa
Source organism: Streptococcus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P19909 (Residues: 303-357; Coverage: 10%)
Gene name: spg
Sequence domains: B domain
Structure domains: Ubiquitin-like (UB roll)

Ligands and Environments

2 bound ligands:
Ligand ZN 6 x ZN
Ligand CL 4 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P32
Unit cell:
a: 48.292Å b: 48.292Å c: 83.341Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.149 0.145 0.214
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 citations in other articles

Biofabrication of functional protein nanoparticles through simple His-tag engineering.
López-Laguna et al. (2021)