› 6nke

X-ray diffraction
1.72Å resolution

Wild-type GGGPS from Thermoplasma volcanium

Released:
DOI: 10.2210/pdb6nke/pdb
PDB 6nke coloured by chain and viewed from the front.
PDB 6nke coloured by chain and viewed from the side.
PDB 6nke coloured by chain and viewed from the top.
Source organism: Thermoplasma volcanium GSS1
Primary publication:
Structural studies of geranylgeranylglyceryl phosphate synthase, a prenyltransferase found in thermophilic Euryarchaeota.
Blank PN, Barnett AA, Ronnebaum TA, Alderfer KE, Gillott BN, Christianson DW, Himmelberger JA
Acta Crystallogr D Struct Biol 76 542-557 (2020)
PMID: 32496216

Function and Biology Details

Reaction catalysed: 
Geranylgeranyl diphosphate + sn-glycerol 1-phosphate = diphosphate + sn-3-O-(geranylgeranyl)glycerol 1-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Geranylgeranylglyceryl phosphate synthase Chain: A
Molecule details ›
Chain: A
Length: 252 amino acids
Theoretical weight: 27.51 KDa
Source organism: Thermoplasma volcanium GSS1
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q97AR4 (Residues: 1-252; Coverage: 100%)
Gene names: TV0745, TVG0750626
Sequence domains: PcrB family
Structure domains: FMN-linked oxidoreductases

Ligands and Environments

3 bound ligands:
Ligand GOL 2 x GOL
Ligand SO4 1 x SO4
Ligand IMD 2 x IMD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: P41212
Unit cell:
a: 73.761Å b: 73.761Å c: 122.6Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.197 0.197 0.204
Expression system: Escherichia coli BL21

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Citations

1 citations in other articles

Hexamerization and thermostability emerged very early during geranylgeranylglyceryl phosphate synthase evolution.
Kropp et al. (2021)