6nil

Electron Microscopy
3.9Å resolution

cryoEM structure of the truncated HIV-1 Vif/CBFbeta/A3F complex

Released:
Primary publication:
Structural basis of antagonism of human APOBEC3F by HIV-1 Vif.
Nat. Struct. Mol. Biol. 26 1176-1183 (2019)
PMID: 31792451
Related structures: EMD-9380

Function and Biology Details

Reaction catalysed:
Cytosine in single-stranded DNA + H(2)O = uracil in single-stranded DNA + NH(3)
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
Non-polymer only dodecamer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
DNA dC->dU-editing enzyme APOBEC-3F Chains: A, D, G, J
Molecule details ›
Chains: A, D, G, J
Length: 207 amino acids
Theoretical weight: 24.43 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8IUX4 (Residues: 185-373; Coverage: 51%)
Gene name: APOBEC3F
Sequence domains: APOBEC-like N-terminal domain
Core-binding factor subunit beta Chains: B, E, H, K
Molecule details ›
Chains: B, E, H, K
Length: 151 amino acids
Theoretical weight: 17.85 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q13951 (Residues: 1-151; Coverage: 83%)
Gene name: CBFB
Virion infectivity factor Chains: C, F, I, L
Molecule details ›
Chains: C, F, I, L
Length: 138 amino acids
Theoretical weight: 16.74 KDa
Source organism: Human immunodeficiency virus 1
Expression system: Escherichia coli
UniProt:
  • Canonical: P12504 (Residues: 1-113, 158-176; Coverage: 69%)
Gene name: vif

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.9Å
Relevant EMDB volumes: EMD-9380
Expression system: Escherichia coli