› 6nfz

X-ray diffraction
2.97Å resolution

Crystal structure of diphosphorylated HPK1 kinase domain in complex with sunitinib in the active state.

Released:
DOI: 10.2210/pdb6nfz/pdb
PDB entry 6nfz coloured by chain, front view.
PDB entry 6nfz coloured by chain, side view.
PDB entry 6nfz coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Multiple conformational states of the HPK1 kinase domain in complex with sunitinib reveal the structural changes accompanying HPK1 trans-regulation.
Johnson E, McTigue M, Gallego RA, Johnson TW, Timofeevski S, Maestre M, Fisher TS, Kania R, Sawasdikosol S, Burakoff S, Cronin CN
J Biol Chem 294 9029-9036 (2019)
PMID: 31018963

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-187814 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Mitogen-activated protein kinase kinase kinase kinase 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 309 amino acids
Theoretical weight: 34.59 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q92918 (Residues: 1-307; Coverage: 37%)
Gene names: HPK1, MAP4K1
Sequence domains: Protein kinase domain

Ligands and Environments

1 bound ligand:
Ligand B49 2 x B49
2 modified residues:
Ligand TPO 2 x TPO
Ligand SEP 2 x SEP

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: R32
Unit cell:
a: 149.932Å b: 149.932Å c: 156.754Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.209 0.207 0.25
Expression system: Spodoptera frugiperda

Quick links

Citations

1 review citation

A perspective on HPK1 as a novel immuno-oncology drug target.
Sawasdikosol et al. (2020)
9 other articles

2 mentions without citation

Multiple conformational states of the HPK1 kinase domain in complex with sunitinib reveal the structural changes accompanying HPK1 trans-regulation.
Johnson et al. (2019)
1 more