6nce

X-ray diffraction
2.6Å resolution

Crystal structure of the human FOXN3 DNA binding domain in complex with a forkhead DNA sequence

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Forkhead box protein N3 Chain: A
Molecule details ›
Chain: A
Length: 100 amino acids
Theoretical weight: 11.68 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O00409 (Residues: 112-210; Coverage: 20%)
Gene names: C14orf116, CHES1, FOXN3
Sequence domains: Forkhead domain
DNA (5'-D(*TP*CP*TP*TP*AP*AP*GP*TP*AP*AP*AP*CP*AP*AP*TP*G)-3') Chain: C
Molecule details ›
Chain: C
Length: 16 nucleotides
Theoretical weight: 4.91 KDa
Source organism: synthetic construct
Expression system: Not provided
DNA (5'-D(*AP*CP*AP*TP*TP*GP*TP*TP*TP*AP*CP*TP*TP*AP*AP*G)-3') Chain: D
Molecule details ›
Chain: D
Length: 16 nucleotides
Theoretical weight: 4.89 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: C2
Unit cell:
a: 94.94Å b: 102.08Å c: 34.45Å
α: 90° β: 102.65° γ: 90°
R-values:
R R work R free
0.235 0.235 0.271
Expression systems:
  • Escherichia coli
  • Not provided