› 6nc7

X-ray diffraction
3Å resolution

Lipid II flippase MurJ, inward open conformation

Released:
DOI: 10.2210/pdb6nc7/pdb
PDB entry 6nc7 coloured by chain, front view.
PDB entry 6nc7 coloured by chain, side view.
PDB entry 6nc7 coloured by chain, top view.
Source organism: Thermosipho africanus TCF52B
Primary publication:
Visualizing conformation transitions of the Lipid II flippase MurJ.
Kuk ACY, Hao A, Guan Z, Lee SY
Nat Commun 10 1736 (2019)
PMID: 30988294

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-110486 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Lipid II flippase MurJ Chains: A, B
Molecule details ›
Chains: A, B
Length: 475 amino acids
Theoretical weight: 54.14 KDa
Source organism: Thermosipho africanus TCF52B
Expression system: Escherichia coli
UniProt:
  • Canonical: B7IE18 (Residues: 1-475; Coverage: 100%)
Gene names: THA_1814, murJ, mviN
Sequence domains: Lipid II flippase MurJ

Ligands and Environments

2 bound ligands:
Ligand OLC 7 x OLC
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: C2
Unit cell:
a: 129.692Å b: 105.514Å c: 111.479Å
α: 90° β: 125.29° γ: 90°
R-values:
R R work R free
0.25 0.249 0.271
Expression system: Escherichia coli

Quick links

Citations

12 review citations

Regulation of peptidoglycan synthesis and remodelling.
Egan et al. (2020)
11 more

3 mentions without citation

The role of π-helices in TRP channel gating.
Zubcevic et al. (2019)
2 more