Structure analysis

Cryo-EM structure of Rpl10-inserted (RI) pre-60S ribosomal subunit

Electron Microscopy
3.5Å resolution
Assembly composition:
hetero 49-mer (preferred)
Entry contents: 46 distinct polypeptide molecules
3 distinct RNA molecules

Assemblies

Assembly 1 (preferred)
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Multimeric state: hetero 49-mer
Accessible surface area: 703341.85 Å2
Buried surface area: 368170.39 Å2
Dissociation area: 1,585.03 Å2
Dissociation energy (ΔGdiss): 10.51 kcal/mol
Dissociation entropy (TΔSdiss): 12.27 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-135769

Macromolecules

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Chain: X
Length: 245 amino acids
Theoretical weight: 26.48 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: Q12522 (Residues: 1-245; Coverage: 100%)
Gene names: CDC95, LPZ15C, TIF6, YPR016C
Pfam: eIF-6 family
InterPro: Translation initiation factor IF6

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Chain: W
Length: 640 amino acids
Theoretical weight: 72.84 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P53145 (Residues: 1-640; Coverage: 100%)
Gene names: LSG1, YGL099W
Pfam: 50S ribosome-binding GTPase
InterPro:

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Chain: V
Length: 518 amino acids
Theoretical weight: 59.17 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P38861 (Residues: 1-518; Coverage: 100%)
Gene names: NMD3, YHR170W
Pfam:
InterPro:

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Chain: N
Length: 199 amino acids
Theoretical weight: 22.6 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: Q12690 (Residues: 1-199; Coverage: 100%)
Gene names: RPL13A, YDL082W
Pfam: Ribosomal protein L13e
InterPro:

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Chain: Q
Length: 106 amino acids
Theoretical weight: 12.25 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P0CX27 (Residues: 1-106; Coverage: 100%)
Gene names: N1722, RPL41A, RPL42A, SCL41A, YNL162W
Pfam: Ribosomal protein L44
InterPro:

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Chain: R
Length: 92 amino acids
Theoretical weight: 10.11 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P0CX25 (Residues: 1-92; Coverage: 100%)
Gene names: RPL43A, YP9499.02, YPR043W
Pfam: Ribosomal L37ae protein family
InterPro:

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Chain: S
Length: 217 amino acids
Theoretical weight: 18.49 KDa
Source organism: Saccharomyces cerevisiae S288C

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Chain: f
Length: 172 amino acids
Theoretical weight: 20.48 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P0CX23 (Residues: 1-172; Coverage: 100%)
Gene names: RPL18A, RPL18A2, RPL20A, YM9408.04C, YMR242C
Pfam: Ribosomal proteins 50S-L18Ae/60S-L20/60S-L18A
InterPro:

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Chain: h
Length: 121 amino acids
Theoretical weight: 13.71 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P05749 (Residues: 1-121; Coverage: 100%)
Gene names: L2168, RPL22A, YLR061W
Pfam: Ribosomal L22e protein family
InterPro:

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Chain: i
Length: 137 amino acids
Theoretical weight: 14.49 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P0CX41 (Residues: 1-137; Coverage: 100%)
Gene names: RPL17A, RPL17AA, RPL23A, YBL0713, YBL087C
Pfam: Ribosomal protein L14p/L23e
InterPro:

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Chain: j
Length: 155 amino acids
Theoretical weight: 17.66 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P04449 (Residues: 1-155; Coverage: 100%)
Gene names: RP29, RPL24A, RPL30A, YGL031C
Pfam: Ribosomal protein L24e
InterPro:

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Chain: o
Length: 59 amino acids
Theoretical weight: 6.69 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P05747 (Residues: 1-59; Coverage: 100%)
Gene names: RPL29, YFR032BW, YFR032C-A, YL43
Pfam: Ribosomal L29e protein family
InterPro: Large ribosomal subunit protein eL29

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Chain: q
Length: 113 amino acids
Theoretical weight: 12.98 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P0C2H8 (Residues: 1-113; Coverage: 100%)
Gene names: D2478, RPL31A, RPL34, RPL34A, YDL075W
Pfam: Ribosomal protein L31e
InterPro:

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Chain: r
Length: 130 amino acids
Theoretical weight: 14.81 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P38061 (Residues: 1-130; Coverage: 100%)
Gene names: RPL32, YBL0838, YBL092W
Pfam: Ribosomal protein L32
InterPro:

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Chain: t
Length: 121 amino acids
Theoretical weight: 13.67 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P87262 (Residues: 1-121; Coverage: 100%)
Gene names: RPL34A, YER056BC, YER056C-A
Pfam: Ribosomal protein L34e
InterPro:

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Chain: v
Length: 100 amino acids
Theoretical weight: 11.15 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P05745 (Residues: 1-100; Coverage: 100%)
Gene names: RPL36A, RPL39A, YM9646.06, YMR194W
Pfam: Ribosomal protein L36e
InterPro:

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Chain: w
Length: 88 amino acids
Theoretical weight: 9.88 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P49166 (Residues: 1-88; Coverage: 100%)
Gene names: L9470.6, RPL35A, RPL37A, YLR185W
Pfam: Ribosomal protein L37e
InterPro:

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Chain: x
Length: 78 amino acids
Theoretical weight: 8.85 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P49167 (Residues: 1-78; Coverage: 100%)
Gene names: L8543.2, RPL38, YLR325C
Pfam: Ribosomal L38e protein family
InterPro:

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Name: Saccharomyces cerevisiae S288C 35S pre-ribosomal RNA (RDN37-1), miscRNA
Representative chains: A
Length: 3396 nucleotides
Theoretical weight: 1097.49 KDa
Rfam: Eukaryotic large subunit ribosomal RNA

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Name: 5S ribosomal RNA
Representative chains: B
Length: 121 nucleotides
Theoretical weight: 38.95 KDa
Rfam: 5S ribosomal RNA

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Name: 5.8S ribosomal RNA
Representative chains: C
Length: 158 nucleotides
Theoretical weight: 50.68 KDa
Rfam: 5.8S ribosomal RNA

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