6n7f

X-ray diffraction
1.9Å resolution

1.90 Angstrom Resolution Crystal Structure of Glutathione Reductase from Streptococcus pyogenes in Complex with FAD.

Released:
Entry authors: Minasov G, Shuvalova L, Shabalin IG, Grabowski M, Olphie A, Cardona-Correa A, Anderson WF, Satchell KJF, Joachimiak A, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putative glutathione reductase (GR) Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 453 amino acids
Theoretical weight: 49.25 KDa
Source organism: Streptococcus pyogenes serotype M1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9A0E2 (Residues: 1-450; Coverage: 100%)
Gene names: SPy_0813, gor
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand FAD 4 x FAD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: P1
Unit cell:
a: 76.4Å b: 86.65Å c: 86.67Å
α: 67.13° β: 74.15° γ: 74.11°
R-values:
R R work R free
0.171 0.169 0.21
Expression system: Escherichia coli BL21(DE3)