X-ray diffraction
3.69Å resolution

Crystal structure of a double Trp XylE mutants (G58W/L315W)


Function and Biology Details

Reaction catalysed:
A n-alkanal + NAD(P)(+) = an alk-2-enal + NAD(P)H
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
D-xylose-proton symporter Chain: A
Molecule details ›
Chain: A
Length: 518 amino acids
Theoretical weight: 56.77 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
  • Canonical: P0AGF4 (Residues: 1-491; Coverage: 100%)
Gene names: JW3991, b4031, xylE
Sequence domains: Sugar (and other) transporter
Structure domains: MFS general substrate transporter like domains

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P212121
Unit cell:
a: 89.076Å b: 95.482Å c: 106.837Å
α: 90° β: 90° γ: 90°
R R work R free
0.293 0.291 0.33
Expression system: Escherichia coli