› 6mze

X-ray diffraction
3.6Å resolution

Structural Basis of Tubulin Recruitment and Assembly by Microtubule Polymerases with Tumor Overexpressed Gene (TOG) Domain Arrays

Released:
DOI: 10.2210/pdb6mze/pdb
PDB entry 6mze coloured by chain, front view.
PDB entry 6mze coloured by chain, side view.
PDB entry 6mze coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis of tubulin recruitment and assembly by microtubule polymerases with tumor overexpressed gene (TOG) domain arrays.
Nithianantham S, Cook BD, Beans M, Guo F, Chang F, Al-Bassam J
Elife 7 (2018)
PMID: 30422110

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetradecamer (preferred)
PDBe Complex ID:
PDB-CPX-105121 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Tubulin alpha-1A chain Chains: A, C, H, J, O, Q, V, X
Molecule details ›
Chains: A, C, H, J, O, Q, V, X
Length: 451 amino acids
Theoretical weight: 50.12 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: P02550 (Residues: 1-451; Coverage: 100%)
Gene name: TUBA1A
Sequence domains:
Structure domains:
Tubulin beta chain Chains: B, D, I, K, P, R, W, Y
Molecule details ›
Chains: B, D, I, K, P, R, W, Y
Length: 445 amino acids
Theoretical weight: 49.91 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: P02554 (Residues: 1-445; Coverage: 100%)
Sequence domains:
Structure domains:
TOG domain-containing protein Chains: E, L, S, Z
Molecule details ›
Chains: E, L, S, Z
Length: 536 amino acids
Theoretical weight: 60.89 KDa
Source organism: Lachancea kluyveri NRRL Y-12651
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: A0A493R6X7 (Residues: 1-536; Coverage: 100%)
Gene name: SKLU-Cont10078
Sequence domains: XMAP215/Dis1/CLASP, TOG domain
Designed ankyrin repeat protein (DARPIN) D1 Chains: F, G, M, N, T, U, a, b
Molecule details ›
Chains: F, G, M, N, T, U, a, b
Length: 169 amino acids
Theoretical weight: 18.07 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21
Structure domains: Ankyrin repeat-containing domain

Ligands and Environments

3 bound ligands:
Ligand GTP 8 x GTP
Ligand MG 16 x MG
Ligand GDP 8 x GDP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P21
Unit cell:
a: 218.48Å b: 106.15Å c: 282.23Å
α: 90° β: 90.39° γ: 90°
R-values:
R R work R free
0.206 0.202 0.245
Expression system: Escherichia coli BL21

Quick links

Citations

5 review citations

The tubulin code and its role in controlling microtubule properties and functions.
Janke et al. (2020)
4 more

1 mention without citation

Structural basis of tubulin recruitment and assembly by microtubule polymerases with tumor overexpressed gene (TOG) domain arrays.
Nithianantham et al. (2018)