› 6mw8

X-ray diffraction
1.76Å resolution

UDP-galactose:glucoside-Skp1 alpha-D-galactosyltransferase with bound UDP and Manganese

Released:
DOI: 10.2210/pdb6mw8/pdb
PDB entry 6mw8 coloured by chain, front view.
PDB entry 6mw8 coloured by chain, side view.
PDB entry 6mw8 coloured by chain, top view.
Source organism: Globisporangium ultimum
Primary publication:
A terminal α3-galactose modification regulates an E3 ubiquitin ligase subunit in Toxoplasma gondii.
Mandalasi M, Kim HW, Thieker D, Sheikh MO, Gas-Pascual E, Rahman K, Zhao P, Daniel NG, van der Wel H, Ichikawa HT, Glushka JN, Wells L, Woods RJ, Wood ZA, West CM
J Biol Chem 295 9223-9243 (2020)
PMID: 32414843

Function and Biology Details

Reaction catalysed: 
UDP-alpha-D-galactose + beta-D-galactosyl-(1->4)-beta-N-acetyl-D-glucosaminyl-R = UDP + alpha-D-galactosyl-(1->3)-beta-D-galactosyl-(1->4)-beta-N-acetylglucosaminyl-R
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-125515 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glycosyl transferase family 8 protein Chain: A
Molecule details ›
Chain: A
Length: 266 amino acids
Theoretical weight: 30.11 KDa
Source organism: Globisporangium ultimum
Expression system: Escherichia coli
UniProt:
  • Canonical: K3WC47 (Residues: 1-266; Coverage: 100%)
Sequence domains: Glycosyl transferase family 8

Ligands and Environments

4 bound ligands:
Ligand MN 1 x MN
Ligand UDP 1 x UDP
Ligand ACT 3 x ACT
Ligand EDO 1 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-BM
Spacegroup: P42212
Unit cell:
a: 84.055Å b: 84.055Å c: 76.076Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.182 0.181 0.208
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Glycomics, Glycoproteomics, and Glycogenomics: An Inter-Taxa Evolutionary Perspective.
West et al. (2021)
2 other articles

1 mention without citation

A terminal α3-galactose modification regulates an E3 ubiquitin ligase subunit in Toxoplasma gondii.
Mandalasi et al. (2020)