PDBe 6mux

Electron Microscopy
3.9Å resolution

The structure of the Plasmodium falciparum 20S proteasome in complex with one PA28 activator

Released:

Function and Biology Details

Reaction catalysed:
Cleavage of peptide bonds with very broad specificity.
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero 35-mer (preferred)
Entry contents:
15 distinct polypeptide molecules
Macromolecules (15 distinct):
Proteasome endopeptidase complex Chains: A, O
Molecule details ›
Chains: A, O
Length: 260 amino acids
Theoretical weight: 29.53 KDa
Source organism: Plasmodium falciparum 3D7
UniProt:
  • Canonical: Q8IAR3 (Residues: 1-260; Coverage: 100%)
Gene name: PF3D7_0807500
Sequence domains:
Proteasome endopeptidase complex Chains: B, P
Molecule details ›
Chains: B, P
Length: 235 amino acids
Theoretical weight: 26.56 KDa
Source organism: Plasmodium falciparum 3D7
UniProt:
  • Canonical: C6KST3 (Residues: 1-235; Coverage: 100%)
Gene name: PF3D7_0608500
Sequence domains:
Proteasome subunit alpha type Chains: C, Q
Molecule details ›
Chains: C, Q
Length: 246 amino acids
Theoretical weight: 27.98 KDa
Source organism: Plasmodium falciparum 3D7
UniProt:
  • Canonical: Q8IDG3 (Residues: 1-246; Coverage: 100%)
Gene name: PF3D7_1353800
Sequence domains:
Proteasome subunit alpha type Chains: D, R
Molecule details ›
Chains: D, R
Length: 241 amino acids
Theoretical weight: 27.26 KDa
Source organism: Plasmodium falciparum 3D7
UniProt:
  • Canonical: Q8IDG2 (Residues: 1-241; Coverage: 100%)
Gene name: PF3D7_1353900
Sequence domains:
Proteasome subunit alpha type Chains: E, S
Molecule details ›
Chains: E, S
Length: 256 amino acids
Theoretical weight: 28.42 KDa
Source organism: Plasmodium falciparum 3D7
UniProt:
  • Canonical: Q8IBI3 (Residues: 1-256; Coverage: 100%)
Gene name: PF3D7_0727400
Sequence domains:
Proteasome endopeptidase complex Chains: F, T
Molecule details ›
Chains: F, T
Length: 254 amino acids
Theoretical weight: 28.87 KDa
Source organism: Plasmodium falciparum 3D7
UniProt:
  • Canonical: Q8IK90 (Residues: 1-254; Coverage: 100%)
Gene name: PF3D7_1474800
Sequence domains:
PROTEASOME_ALPHA_1 domain-containing protein Chains: G, U
Molecule details ›
Chains: G, U
Length: 252 amino acids
Theoretical weight: 29.32 KDa
Source organism: Plasmodium falciparum 3D7
UniProt:
  • Canonical: O77396 (Residues: 1-252; Coverage: 100%)
Gene name: PF3D7_0317000
Sequence domains:
Proteasome subunit beta type-6, putative Chains: H, V
Molecule details ›
Chains: H, V
Length: 252 amino acids
Theoretical weight: 29.14 KDa
Source organism: Plasmodium falciparum 3D7
UniProt:
  • Canonical: Q8I0U7 (Residues: 31-282; Coverage: 89%)
Gene name: PF3D7_0931800
Sequence domains: Proteasome subunit
Proteasome subunit beta Chains: I, W
Molecule details ›
Chains: I, W
Length: 229 amino acids
Theoretical weight: 25.1 KDa
Source organism: Plasmodium falciparum 3D7
UniProt:
  • Canonical: Q8I6T3 (Residues: 42-270; Coverage: 85%)
Gene name: PF3D7_1328100
Proteasome subunit beta Chains: J, X
Molecule details ›
Chains: J, X
Length: 218 amino acids
Theoretical weight: 24.53 KDa
Source organism: Plasmodium falciparum 3D7
UniProt:
  • Canonical: Q8I261 (Residues: 1-218; Coverage: 100%)
Gene name: PF3D7_0108000
Sequence domains: Proteasome subunit
Proteasome subunit beta Chains: K, Y
Molecule details ›
Chains: K, Y
Length: 195 amino acids
Theoretical weight: 22.89 KDa
Source organism: Plasmodium falciparum 3D7
UniProt:
  • Canonical: Q8IKC9 (Residues: 1-195; Coverage: 100%)
Gene name: PF3D7_1470900
Sequence domains: Proteasome subunit
Proteasome subunit beta Chains: L, Z
Molecule details ›
Chains: L, Z
Length: 211 amino acids
Theoretical weight: 23.62 KDa
Source organism: Plasmodium falciparum 3D7
UniProt:
  • Canonical: Q8IJT1 (Residues: 61-271; Coverage: 78%)
Gene name: PF3D7_1011400
Proteasome subunit beta Chains: M, a
Molecule details ›
Chains: M, a
Length: 240 amino acids
Theoretical weight: 27.3 KDa
Source organism: Plasmodium falciparum 3D7
UniProt:
  • Canonical: C0H4E8 (Residues: 1-240; Coverage: 100%)
Gene name: PF3D7_0518300
Sequence domains: Proteasome subunit
Proteasome subunit beta Chains: N, b
Molecule details ›
Chains: N, b
Length: 265 amino acids
Theoretical weight: 30.91 KDa
Source organism: Plasmodium falciparum 3D7
UniProt:
  • Canonical: Q7K6A9 (Residues: 1-265; Coverage: 100%)
Gene name: PF3D7_0803800
Sequence domains: Proteasome subunit
PA28_beta domain-containing protein Chains: c, d, e, f, g, h, i
Molecule details ›
Chains: c, d, e, f, g, h, i
Length: 279 amino acids
Theoretical weight: 33.18 KDa
Source organism: Plasmodium falciparum 3D7
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8I374 (Residues: 1-279; Coverage: 100%)
Gene name: PF3D7_0907700
Sequence domains: Proteasome activator pa28 beta subunit

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.9Å
Relevant EMDB volumes: EMD-9259
Expression system: Escherichia coli BL21(DE3)