› › Structure analysis

Cryo-EM structure of Csm-crRNA-target RNA ternary complex in type III-A CRISPR-Cas system

Electron Microscopy
3.1Å resolution
PDB entry 6mur coloured by chain, front view.
PDB entry 6mur coloured by chain, side view.
PDB entry 6mur coloured by chain, top view.
Source organisms:
Assembly composition:
hetero octamer (preferred)
Entry contents: 5 distinct polypeptide molecules
2 distinct RNA molecules

Quick links

Assemblies

Assembly 1 (preferred)
Download    3D Visualisation
Multimeric state: hetero octamer
Accessible surface area: 83608.14 Å2
Buried surface area: 33847.56 Å2
Dissociation area: 1,821.64 Å2
Dissociation energy (ΔGdiss): 15.73 kcal/mol
Dissociation entropy (TΔSdiss): 13.25 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-110419

Macromolecules

CRISPR system single-strand-specific deoxyribonuclease Cas10/Csm1 (subtype III-A)
Chain: A
Length: 791 amino acids
Theoretical weight: 89.68 KDa
Source organism: Thermococcus onnurineus NA1
Expression system: Escherichia coli
UniProt:
  • Canonical: B6YWB8 (Residues: 1-777; Coverage: 100%)
Gene names: TON_0893, cas10, csm1
Pfam:
InterPro:
CRISPR system single-strand-specific deoxyribonuclease Cas10/Csm1 (subtype III-A) in PDB entry 6mur, assembly 1, front view.
CRISPR system single-strand-specific deoxyribonuclease Cas10/Csm1 (subtype III-A) in PDB entry 6mur, assembly 1, side view.
CRISPR system single-strand-specific deoxyribonuclease Cas10/Csm1 (subtype III-A) in PDB entry 6mur, assembly 1, top view.
Molecule details ›

Search similar proteins

CRISPR system Cms protein Csm2
Chain: B
Length: 187 amino acids
Theoretical weight: 21.21 KDa
Source organism: Thermococcus onnurineus NA1
Expression system: Escherichia coli
UniProt:
  • Canonical: B6YWB9 (Residues: 1-186; Coverage: 100%)
Gene name: TON_0894
Pfam: Csm2 Type III-A
InterPro: CRISPR-associated protein, Csm2 Type III-A
CRISPR system Cms protein Csm2 in PDB entry 6mur, assembly 1, front view.
CRISPR system Cms protein Csm2 in PDB entry 6mur, assembly 1, side view.
CRISPR system Cms protein Csm2 in PDB entry 6mur, assembly 1, top view.
Molecule details ›

Search similar proteins

CRISPR system Cms endoribonuclease Csm3
Chains: C, D
Length: 291 amino acids
Theoretical weight: 32.77 KDa
Source organism: Thermococcus onnurineus
Expression system: Escherichia coli
UniProt:
  • Canonical: B6YWC0 (Residues: 1-290; Coverage: 100%)
Gene name: TON_0895
Pfam: RAMP superfamily
InterPro:
CRISPR system Cms endoribonuclease Csm3 in PDB entry 6mur, assembly 1, front view.
CRISPR system Cms endoribonuclease Csm3 in PDB entry 6mur, assembly 1, side view.
CRISPR system Cms endoribonuclease Csm3 in PDB entry 6mur, assembly 1, top view.
Molecule details ›

Search similar proteins

CRISPR system Cms protein Csm4
Chain: E
Length: 289 amino acids
Theoretical weight: 32.35 KDa
Source organism: Thermococcus onnurineus NA1
Expression system: Escherichia coli
UniProt:
  • Canonical: B6YWC1 (Residues: 1-289; Coverage: 100%)
Gene names: TON_0896, csm4
InterPro: CRISPR type III-associated RAMP protein Csm4
CRISPR system Cms protein Csm4 in PDB entry 6mur, assembly 1, front view.
CRISPR system Cms protein Csm4 in PDB entry 6mur, assembly 1, side view.
CRISPR system Cms protein Csm4 in PDB entry 6mur, assembly 1, top view.
Molecule details ›

Search similar proteins

CRISPR system Cms protein Csm5
Chain: F
Length: 403 amino acids
Theoretical weight: 46.09 KDa
Source organism: Thermococcus onnurineus NA1
Expression system: Escherichia coli
UniProt:
  • Canonical: B6YWC2 (Residues: 1-397; Coverage: 100%)
Gene name: TON_0897
Pfam: RAMP superfamily
InterPro:
CRISPR system Cms protein Csm5 in PDB entry 6mur, assembly 1, front view.
CRISPR system Cms protein Csm5 in PDB entry 6mur, assembly 1, side view.
CRISPR system Cms protein Csm5 in PDB entry 6mur, assembly 1, top view.
Molecule details ›

Search similar proteins

RNA chain G
Name: RNA (27-MER)
Representative chains: G
Length: 38 nucleotides
Theoretical weight: 12.46 KDa
RNA (27-MER) in PDB entry 6mur, assembly 1, front view.
RNA (27-MER) in PDB entry 6mur, assembly 1, side view.
RNA (27-MER) in PDB entry 6mur, assembly 1, top view.
Molecule details ›

Search similar RNA

RNA chain H
Name: RNA (5'-R(P*GP*CP*AP*AP*AP*CP*CP*GP*CP*CP*UP*CP*UP*GP*CP*CP*CP*GP*C)-3')
Representative chains: H
Length: 40 nucleotides
Theoretical weight: 12.75 KDa
RNA (5'-R(P*GP*CP*AP*AP*AP*CP*CP*GP*CP*CP*UP*CP*UP*GP*CP*CP*CP*GP*C)-3') in PDB entry 6mur, assembly 1, front view.
RNA (5'-R(P*GP*CP*AP*AP*AP*CP*CP*GP*CP*CP*UP*CP*UP*GP*CP*CP*CP*GP*C)-3') in PDB entry 6mur, assembly 1, side view.
RNA (5'-R(P*GP*CP*AP*AP*AP*CP*CP*GP*CP*CP*UP*CP*UP*GP*CP*CP*CP*GP*C)-3') in PDB entry 6mur, assembly 1, top view.
Molecule details ›

Search similar RNA

Quick links