6mgg

X-ray diffraction
1.78Å resolution

Succinyl-CoA synthase from Francisella tularensis, phosphorylated, in complex with CoA

Released:
Entry authors: Osipiuk J, Maltseva N, Jedrzejczak R, Satchell KJF, Joachimiak A, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Succinate--CoA ligase [ADP-forming] subunit alpha Chains: A, C
Molecule details ›
Chains: A, C
Length: 296 amino acids
Theoretical weight: 31.03 KDa
Source organism: Francisella tularensis subsp. tularensis SCHU S4
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5NHF4 (Residues: 1-290; Coverage: 100%)
Gene names: FTT_0503c, sucD
Sequence domains:
Structure domains:
Succinate--CoA ligase [ADP-forming] subunit beta Chains: B, D
Molecule details ›
Chains: B, D
Length: 387 amino acids
Theoretical weight: 41.62 KDa
Source organism: Francisella tularensis subsp. tularensis SCHU S4
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5NHF3 (Residues: 1-387; Coverage: 100%)
Gene names: FTT_0504c, sucC
Sequence domains:

Ligands and Environments


Cofactor: Ligand COA 2 x COA
2 bound ligands:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: P21
Unit cell:
a: 66.911Å b: 158.338Å c: 73.585Å
α: 90° β: 111.73° γ: 90°
R-values:
R R work R free
0.178 0.176 0.214
Expression system: Escherichia coli BL21(DE3)