› 6mcu

X-ray diffraction
2.57Å resolution

Crystal structure of the holo retinal-bound domain-swapped dimer Q108K:T51D:A28H mutant of human Cellular Retinol Binding Protein II

Released:
DOI: 10.2210/pdb6mcu/pdb
PDB entry 6mcu coloured by chain, front view.
PDB entry 6mcu coloured by chain, side view.
PDB entry 6mcu coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Engineering the hCRBPII Domain-Swapped Dimer into a New Class of Protein Switches.
Ghanbarpour A, Pinger C, Esmatpour Salmani R, Assar Z, Santos EM, Nosrati M, Pawlowski K, Spence D, Vasileiou C, Jin X, Borhan B, Geiger JH
J Am Chem Soc 141 17125-17132 (2019)
PMID: 31557439

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156130 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Retinol-binding protein 2 Chains: A, B, C, D, E, F, G, H, I, J, K, L
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L
Length: 133 amino acids
Theoretical weight: 15.68 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P50120 (Residues: 2-134; Coverage: 99%)
Gene names: CRBP2, RBP2
Sequence domains: Lipocalin / cytosolic fatty-acid binding protein family

Ligands and Environments

2 bound ligands:
Ligand GOL 3 x GOL
Ligand RET 5 x RET
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P212121
Unit cell:
a: 65.874Å b: 73.992Å c: 349.989Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.233 0.231 0.296
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Protein Assembly by Design.
Zhu et al. (2021)
5 other articles

1 mention without citation

Engineering the hCRBPII Domain-Swapped Dimer into a New Class of Protein Switches.
Ghanbarpour et al. (2019)