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CryoEM structure of AcrIIA2 homolog in complex with CRISPR-Cas9

Electron Microscopy
3.9Å resolution
PDB entry 6mcc coloured by chain, front view.
PDB entry 6mcc coloured by chain, side view.
PDB entry 6mcc coloured by chain, top view.
Source organisms:
Assembly composition:
hetero trimer (preferred)
Entry contents: 2 distinct polypeptide molecules
1 distinct RNA molecule

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Assemblies

Assembly 1 (preferred)
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Assembly Name: Anti-CRISPR AcrIIA2 Homolog and CRISPR-associated endonuclease Cas9 and RNA
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Multimeric state: hetero trimer
Accessible surface area: 85655.43 Å2
Buried surface area: 17328.0 Å2
Dissociation area: 1,411.03 Å2
Dissociation energy (ΔGdiss): 2.48 kcal/mol
Dissociation entropy (TΔSdiss): 12.96 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-105217

Macromolecules

CRISPR-associated endonuclease Cas9
Chain: A
Length: 1368 amino acids
Theoretical weight: 158.69 KDa
Source organism: Streptococcus pyogenes M1 GAS
Expression system: Escherichia coli
UniProt:
  • Canonical: J7M7J1 (Residues: 1-1368; Coverage: 100%)
Gene names: M1GAS476_0830, cas9
Pfam:
InterPro:
CRISPR-associated endonuclease Cas9 in PDB entry 6mcc, assembly 1, front view.
CRISPR-associated endonuclease Cas9 in PDB entry 6mcc, assembly 1, side view.
CRISPR-associated endonuclease Cas9 in PDB entry 6mcc, assembly 1, top view.
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Anti-CRISPR AcrIIA2 Homolog
Chain: C
Length: 130 amino acids
Theoretical weight: 15.3 KDa
Source organism: Listeria phage LP-101
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A4V8H027 (Residues: 1-130; Coverage: 100%)
Anti-CRISPR AcrIIA2 Homolog in PDB entry 6mcc, assembly 1, front view.
Anti-CRISPR AcrIIA2 Homolog in PDB entry 6mcc, assembly 1, side view.
Anti-CRISPR AcrIIA2 Homolog in PDB entry 6mcc, assembly 1, top view.
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RNA chain B
Name: Single guide RNA (116-MER)
Representative chains: B
Length: 116 nucleotides
Theoretical weight: 37.64 KDa
Rfam: Trans-activating crRNA
Single guide RNA (116-MER) in PDB entry 6mcc, assembly 1, front view.
Single guide RNA (116-MER) in PDB entry 6mcc, assembly 1, side view.
Single guide RNA (116-MER) in PDB entry 6mcc, assembly 1, top view.
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