Electron Microscopy
3.4Å resolution

CryoEM structure of AcrIIA2 in complex with CRISPR-Cas9


Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
2 distinct polypeptide molecules
1 distinct RNA molecule
Macromolecules (3 distinct):
CRISPR-associated endonuclease Cas9 Chain: A
Molecule details ›
Chain: A
Length: 1368 amino acids
Theoretical weight: 158.69 KDa
Source organism: Streptococcus pyogenes M1 GAS
Expression system: Escherichia coli
  • Canonical: J7M7J1 (Residues: 1-1368; Coverage: 100%)
Gene names: M1GAS476_0830, cas9
Sequence domains:
Anti-CRISPR protein AcrIIA2 Chain: C
Molecule details ›
Chain: C
Length: 123 amino acids
Theoretical weight: 14.17 KDa
Source organism: Listeria phage LP-101
Expression system: Escherichia coli
Single guide RNA (116-MER) Chain: B
Molecule details ›
Chain: B
Length: 116 nucleotides
Theoretical weight: 37.64 KDa
Sequence domains: Trans-activating crRNA

Ligands and Environments

No bound ligands
1 modified residue:

Experiments and Validation Details

Entry percentile scores
Resolution: 3.4Å
Relevant EMDB volumes: EMD-9066
Expression system: Escherichia coli