X-ray diffraction
2.45Å resolution

Crystal structure of SARS-CoV-2 spike receptor-binding domain bound with ACE2


Function and Biology Details

Reaction catalysed:
Angiotensin II + H(2)O = angiotensin-(1-7) + L-phenylalanine
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
Non-polymer only dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Processed angiotensin-converting enzyme 2 Chain: A
Molecule details ›
Chain: A
Length: 603 amino acids
Theoretical weight: 69.98 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
  • Canonical: Q9BYF1 (Residues: 19-615; Coverage: 76%)
Gene names: ACE2, UNQ868/PRO1885
Sequence domains: Angiotensin-converting enzyme
Spike protein S1 Chain: E
Molecule details ›
Chain: E
Length: 229 amino acids
Theoretical weight: 25.95 KDa
Source organism: Severe acute respiratory syndrome coronavirus 2
Expression system: Trichoplusia ni
  • Canonical: P0DTC2 (Residues: 319-541; Coverage: 18%)
Gene names: 2, S
Sequence domains: Betacoronavirus spike glycoprotein S1, receptor binding

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P41212
Unit cell:
a: 104.67Å b: 104.67Å c: 228.72Å
α: 90° β: 90° γ: 90°
R R work R free
0.194 0.192 0.227
Expression system: Trichoplusia ni