6lzg

X-ray diffraction
2.5Å resolution

Structure of novel coronavirus spike receptor-binding domain complexed with its receptor ACE2

Released:

Function and Biology Details

Reaction catalysed:
Angiotensin II + H(2)O = angiotensin-(1-7) + L-phenylalanine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Processed angiotensin-converting enzyme 2 Chain: A
Molecule details ›
Chain: A
Length: 596 amino acids
Theoretical weight: 69.04 KDa
Source organism: Homo sapiens
Expression system: Insect cell expression vector pTIE1
UniProt:
  • Canonical: Q9BYF1 (Residues: 19-614; Coverage: 76%)
Gene names: ACE2, UNQ868/PRO1885
Sequence domains: Angiotensin-converting enzyme
Spike protein S1 Chain: B
Molecule details ›
Chain: B
Length: 209 amino acids
Theoretical weight: 23.51 KDa
Source organism: Severe acute respiratory syndrome coronavirus 2
Expression system: Insect cell expression vector pTIE1
UniProt:
  • Canonical: P0DTC2 (Residues: 319-527; Coverage: 17%)
Gene names: 2, S
Sequence domains: Betacoronavirus spike glycoprotein S1, receptor binding

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U1
Spacegroup: P41212
Unit cell:
a: 104.448Å b: 104.448Å c: 229.786Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.19 0.188 0.216
Expression system: Insect cell expression vector pTIE1