6lz9

X-ray diffraction
2.8Å resolution

t8E4 antibody Fab complexed with the active form of HGF

Released:
Source organisms:
Entry authors: Kitago Y, Umitsu M, Takagi J

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Hepatocyte growth factor alpha chain Chain: A
Molecule details ›
Chain: A
Length: 108 amino acids
Theoretical weight: 12.28 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P14210 (Residues: 388-494; Coverage: 15%)
Gene names: HGF, HPTA
Sequence domains: Kringle domain
Hepatocyte growth factor beta chain Chain: B
Molecule details ›
Chain: B
Length: 242 amino acids
Theoretical weight: 27.11 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P14210 (Residues: 495-728; Coverage: 34%)
Gene names: HGF, HPTA
Sequence domains: Trypsin
Heavy chain of t8E4 Fab fragment Chain: H
Molecule details ›
Chain: H
Length: 223 amino acids
Theoretical weight: 24.08 KDa
Source organism: Mus musculus
Expression system: hybrid
Light chain of t8E4 Fab fragment Chain: L
Molecule details ›
Chain: L
Length: 214 amino acids
Theoretical weight: 23.85 KDa
Source organism: Mus musculus
Expression system: hybrid

Ligands and Environments

No bound ligands
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P6322
Unit cell:
a: 176.18Å b: 176.18Å c: 122.96Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.251 0.248 0.298
Expression systems:
  • Homo sapiens
  • hybrid