6lyv

X-ray diffraction
2.7Å resolution

The crystal structure of SAUGI/KSHVUDG complex

Released:

Function and Biology Details

Reaction catalysed:
Hydrolyzes single-stranded DNA or mismatched double-stranded DNA and polynucleotides, releasing free uracil
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Uracil-DNA glycosylase Chains: A, C, E, G, I, K, M, O
Molecule details ›
Chains: A, C, E, G, I, K, M, O
Length: 255 amino acids
Theoretical weight: 29.05 KDa
Source organism: Human gammaherpesvirus 8
Expression system: Escherichia coli
UniProt:
  • Canonical: F5HFA1 (Residues: 1-255; Coverage: 100%)
Gene name: ORF46
Sequence domains: Uracil DNA glycosylase superfamily
SAUGI Chains: B, D, F, H, J, L, N, P
Molecule details ›
Chains: B, D, F, H, J, L, N, P
Length: 112 amino acids
Theoretical weight: 13.37 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q936H5 (Residues: 1-112; Coverage: 100%)
Sequence domains: S. aureus uracil DNA glycosylase inhibitor

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL13C1
Spacegroup: P1
Unit cell:
a: 85.887Å b: 92.196Å c: 98.107Å
α: 108.141° β: 90.612° γ: 92.229°
R-values:
R R work R free
0.194 0.192 0.232
Expression system: Escherichia coli