6lxw

Electron Microscopy
3.27Å resolution

Cryo-EM structure of human secretory immunoglobulin A in complex with the N-terminal domain of SpsA

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero heptamer (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Immunoglobulin heavy constant alpha 1; Interleukin-2 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 291 amino acids
Theoretical weight: 31.45 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P01876 (Residues: 122-353; Coverage: 66%)
  • Canonical: P60568 (Residues: 1-21; Coverage: 1%)
Gene names: IGHA1, IL2
Sequence domains: Immunoglobulin C1-set domain
Immunoglobulin J chain Chain: J
Molecule details ›
Chain: J
Length: 167 amino acids
Theoretical weight: 19.23 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P01591 (Residues: 1-159; Coverage: 100%)
Gene names: IGCJ, IGJ, JCHAIN
Sequence domains: Immunoglobulin J chain
Secretory component Chain: P
Molecule details ›
Chain: P
Length: 573 amino acids
Theoretical weight: 63.31 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P01833 (Residues: 1-565; Coverage: 73%)
Gene name: PIGR
Sequence domains: Immunoglobulin V-set domain
SigA binding protein Chain: S
Molecule details ›
Chain: S
Length: 317 amino acids
Theoretical weight: 36.31 KDa
Source organism: Streptococcus pneumoniae
Expression system: Escherichia phage Ecwhy_1
UniProt:
  • Canonical: O33753 (Residues: 38-324; Coverage: 59%)
Gene name: spsA
Sequence domains: RICH domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.27Å
Relevant EMDB volumes: EMD-30008
Expression systems:
  • Homo sapiens
  • Escherichia phage Ecwhy_1