› 6lxm

X-ray diffraction
2.41Å resolution

Crystal structure of C-terminal DNA-binding domain of Escherichia coli OmpR as a domain-swapped dimer

Released:
DOI: 10.2210/pdb6lxm/pdb
PDB entry 6lxm coloured by chain, front view.
PDB entry 6lxm coloured by chain, side view.
PDB entry 6lxm coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structural basis for promoter DNA recognition by the response regulator OmpR.
Sadotra S, Lou YC, Tang HC, Chiu YC, Hsu CH, Chen C
J Struct Biol 213 107638 (2021)
PMID: 33152421

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141952 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA-binding dual transcriptional regulator OmpR Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 113 amino acids
Theoretical weight: 13.1 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0AA16 (Residues: 136-239; Coverage: 44%)
Gene names: JW3368, b3405, kmt, ompB, ompR
Sequence domains: Transcriptional regulatory protein, C terminal

Ligands and Environments

3 bound ligands:
Ligand BTB 1 x BTB
Ligand SO4 6 x SO4
Ligand GOL 9 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL13C1
Spacegroup: P3121
Unit cell:
a: 98.683Å b: 98.683Å c: 92.717Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.186 0.182 0.221
Expression system: Escherichia coli BL21(DE3)

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Citations

6 citation in other articles

CovS inactivation reduces CovR promoter binding at diverse virulence factor encoding genes in group A Streptococcus.
Horstmann et al. (2022)
5 more