› 6lqp

Electron Microscopy
3.2Å resolution

Cryo-EM structure of 90S small subunit preribosomes in transition states (State A)

Released:
DOI: 10.2210/pdb6lqp/pdb
PDB entry 6lqp coloured by chain, front view.
PDB entry 6lqp coloured by chain, side view.
PDB entry 6lqp coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
Cryo-EM structure of 90S small ribosomal subunit precursors in transition states.
Du Y, An W, Zhu X, Sun Q, Qi J, Ye K
Science 369 1477-1481 (2020)
PMID: 32943522
Related structures: EMD-0949

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine + pseudouridine(1191) in yeast 18S rRNA = S-adenosyl-L-homocysteine + N(1)-methylpseudouridine(1191) in yeast 18S rRNA
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 73-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-126871 (preferred)
Entry contents:
66 distinct polypeptide molecules
3 distinct RNA molecules
Macromolecules (69 distinct):
Small ribosomal subunit protein eS1A Chain: SC
Molecule details ›
Chain: SC
Length: 255 amino acids
Theoretical weight: 28.8 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P33442 (Residues: 1-255; Coverage: 100%)
Gene names: L9753.9, PLC1, RPS10A, RPS1A, YLR441C
Sequence domains: Ribosomal S3Ae family
Small ribosomal subunit protein eS4A Chain: SF
Molecule details ›
Chain: SF
Length: 261 amino acids
Theoretical weight: 29.47 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P0CX35 (Residues: 1-261; Coverage: 100%)
Gene names: J2186, RPS4A, RPS7B, YJR145C
Sequence domains:
Small ribosomal subunit protein uS7 Chain: SG
Molecule details ›
Chain: SG
Length: 225 amino acids
Theoretical weight: 25.07 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P26783 (Residues: 1-225; Coverage: 100%)
Gene names: J2045, RPS2, RPS5, YJR123W
Sequence domains: Ribosomal protein S7p/S5e
Small ribosomal subunit protein eS6A Chain: SH
Molecule details ›
Chain: SH
Length: 236 amino acids
Theoretical weight: 27.05 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P0CX37 (Residues: 1-236; Coverage: 100%)
Gene names: LPG18C, RPS101, RPS10B, RPS6A, YPL090C
Sequence domains: Ribosomal protein S6e
Small ribosomal subunit protein eS7A Chain: SI
Molecule details ›
Chain: SI
Length: 190 amino acids
Theoretical weight: 21.66 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P26786 (Residues: 1-190; Coverage: 100%)
Gene names: RPS30, RPS7A, YOR096W, YOR3177W
Sequence domains: Ribosomal protein S7e
Small ribosomal subunit protein eS8A Chain: SJ
Molecule details ›
Chain: SJ
Length: 200 amino acids
Theoretical weight: 22.54 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P0CX39 (Residues: 1-200; Coverage: 100%)
Gene names: RPS14A, RPS8A, YBL06.05, YBL0613, YBL072C
Sequence domains: Ribosomal protein S8e
Small ribosomal subunit protein uS4A Chain: SK
Molecule details ›
Chain: SK
Length: 197 amino acids
Theoretical weight: 22.49 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: O13516 (Residues: 1-197; Coverage: 100%)
Gene names: RPS13A, RPS9A, YPL081W, YS11A
Sequence domains:
Small ribosomal subunit protein uS17A Chain: SM
Molecule details ›
Chain: SM
Length: 156 amino acids
Theoretical weight: 17.79 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P0CX47 (Residues: 1-156; Coverage: 100%)
Gene names: RPS11A, RPS18A, YD9813.03, YDR025W
Sequence domains:
Small ribosomal subunit protein eS12 Chain: SN
Molecule details ›
Chain: SN
Length: 143 amino acids
Theoretical weight: 15.49 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P48589 (Residues: 1-143; Coverage: 100%)
Gene names: RPS12, YOR369C
Sequence domains: Ribosomal protein L7Ae/L30e/S12e/Gadd45 family
Small ribosomal subunit protein uS15 Chain: SO
Molecule details ›
Chain: SO
Length: 151 amino acids
Theoretical weight: 17.06 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P05756 (Residues: 1-151; Coverage: 100%)
Gene names: D4252, RPS13, RPS13C, YD9609.18, YDR064W
Sequence domains:
Small ribosomal subunit protein uS11A Chain: SP
Molecule details ›
Chain: SP
Length: 137 amino acids
Theoretical weight: 14.56 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P06367 (Residues: 1-137; Coverage: 100%)
Gene names: CRY1, RPL59, RPS14A, YCR031C, YCR31C
Sequence domains: Ribosomal protein S11
Small ribosomal subunit protein uS9A Chain: SR
Molecule details ›
Chain: SR
Length: 143 amino acids
Theoretical weight: 15.88 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P0CX51 (Residues: 1-143; Coverage: 100%)
Gene names: RP61R, RPS16A, YM9375.12, YMR143W
Sequence domains: Ribosomal protein S9/S16
Small ribosomal subunit protein uS13A Chain: ST
Molecule details ›
Chain: ST
Length: 146 amino acids
Theoretical weight: 17.07 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P0CX55 (Residues: 1-146; Coverage: 100%)
Gene names: D9461.35, RPS18A, YDR450W
Sequence domains: Ribosomal protein S13/S18
Small ribosomal subunit protein uS8B Chain: SX
Molecule details ›
Chain: SX
Length: 130 amino acids
Theoretical weight: 14.65 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: Q3E7Y3 (Residues: 1-130; Coverage: 100%)
Gene names: L8039.6, RPS22B, RPS24B, YLR367W
Sequence domains: Ribosomal protein S8
Small ribosomal subunit protein uS12A Chain: SY
Molecule details ›
Chain: SY
Length: 145 amino acids
Theoretical weight: 16.07 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P0CX29 (Residues: 1-145; Coverage: 100%)
Gene names: G6178, RPS23A, RPS28A, YGR118W
Sequence domains: Ribosomal protein S12/S23
Small ribosomal subunit protein eS24A Chain: SZ
Molecule details ›
Chain: SZ
Length: 135 amino acids
Theoretical weight: 15.36 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P0CX31 (Residues: 1-135; Coverage: 100%)
Gene names: RPS24A, RPS24EA, YER074W
Sequence domains: Ribosomal protein S24e
Small ribosomal subunit protein eS27A Chain: Sc
Molecule details ›
Chain: Sc
Length: 82 amino acids
Theoretical weight: 8.89 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P35997 (Residues: 1-82; Coverage: 100%)
Gene names: RPS27A, YKL156W
Sequence domains: Ribosomal protein S27
Small ribosomal subunit protein eS28A Chain: Sd
Molecule details ›
Chain: Sd
Length: 67 amino acids
Theoretical weight: 7.61 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: Q3E7X9 (Residues: 1-67; Coverage: 100%)
Gene names: O3600, RPS28A, RPS33, RPS33A, YOR167C
Sequence domains: Ribosomal protein S28e
rRNA 2'-O-methyltransferase fibrillarin Chains: 3B, 3C
Molecule details ›
Chains: 3B, 3C
Length: 327 amino acids
Theoretical weight: 34.53 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P15646 (Residues: 1-327; Coverage: 100%)
Gene names: D2870, LOT3, NOP1, YDL014W
Sequence domains: Fibrillarin
Nucleolar protein 56 Chain: 3D
Molecule details ›
Chain: 3D
Length: 504 amino acids
Theoretical weight: 56.96 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: Q12460 (Residues: 1-504; Coverage: 100%)
Gene names: L8167.9, NOP56, SIK1, YLR197W
Sequence domains:
Nucleolar protein 58 Chain: 3E
Molecule details ›
Chain: 3E
Length: 511 amino acids
Theoretical weight: 57.06 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: Q12499 (Residues: 1-511; Coverage: 100%)
Gene names: NOP5, NOP58, O6108, YOR310C
Sequence domains:
Ribosomal RNA-processing protein 9 Chain: 3F
Molecule details ›
Chain: 3F
Length: 573 amino acids
Theoretical weight: 65.15 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: Q06506 (Residues: 1-573; Coverage: 100%)
Gene names: RRP9, YPR137W
Sequence domains: WD domain, G-beta repeat
13 kDa ribonucleoprotein-associated protein Chains: 3G, 3H
Molecule details ›
Chains: 3G, 3H
Length: 126 amino acids
Theoretical weight: 13.58 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P39990 (Residues: 1-126; Coverage: 100%)
Gene names: SNU13, YEL026W
Sequence domains: Ribosomal protein L7Ae/L30e/S12e/Gadd45 family
U3 small nucleolar RNA-associated protein 4 Chain: A4
Molecule details ›
Chain: A4
Length: 776 amino acids
Theoretical weight: 87.91 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: Q06679 (Residues: 1-776; Coverage: 100%)
Gene names: UTP4, YDR324C
Sequence domains: WD domain, G-beta repeat
U3 small nucleolar RNA-associated protein 5 Chain: A5
Molecule details ›
Chain: A5
Length: 643 amino acids
Theoretical weight: 72.08 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: Q04177 (Residues: 1-643; Coverage: 100%)
Gene names: D9509.17, UTP5, YDR398W
Sequence domains: Dip2/Utp12 Family
U3 small nucleolar RNA-associated protein 8 Chain: A8
Molecule details ›
Chain: A8
Length: 713 amino acids
Theoretical weight: 80.27 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P53276 (Residues: 1-713; Coverage: 100%)
Gene names: UTP8, YGR128C
Sequence domains:
U3 small nucleolar RNA-associated protein 9 Chain: A9
Molecule details ›
Chain: A9
Length: 575 amino acids
Theoretical weight: 65.35 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P38882 (Residues: 1-575; Coverage: 100%)
Gene names: UTP9, YHR196W
U3 small nucleolar RNA-associated protein 10 Chain: AE
Molecule details ›
Chain: AE
Length: 1769 amino acids
Theoretical weight: 200.3 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P42945 (Residues: 1-1769; Coverage: 100%)
Gene names: J0808, UTP10, YJL109C
Sequence domains:
U3 small nucleolar RNA-associated protein 15 Chain: AF
Molecule details ›
Chain: AF
Length: 513 amino acids
Theoretical weight: 57.77 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: Q04305 (Residues: 1-513; Coverage: 100%)
Gene names: UTP15, YM9582.18, YMR093W
Sequence domains:
NET1-associated nuclear protein 1 Chain: AG
Molecule details ›
Chain: AG
Length: 896 amino acids
Theoretical weight: 101.34 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: Q02931 (Residues: 1-896; Coverage: 100%)
Gene names: NAN1, UTP17, YPL126W
Sequence domains: WD domain, G-beta repeat
Periodic tryptophan protein 2 Chain: B1
Molecule details ›
Chain: B1
Length: 923 amino acids
Theoretical weight: 104.1 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P25635 (Residues: 1-923; Coverage: 100%)
Gene names: PWP2, UTP1, YCR057C, YCR55C/YCR57C/YCR58C
Sequence domains:
U3 small nucleolar RNA-associated protein 12 Chain: B2
Molecule details ›
Chain: B2
Length: 943 amino acids
Theoretical weight: 106.48 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: Q12220 (Residues: 1-943; Coverage: 100%)
Gene names: DIP2, L3116, L9233.1, UTP12, YLR129W
Sequence domains:
U3 small nucleolar RNA-associated protein 13 Chain: B3
Molecule details ›
Chain: B3
Length: 817 amino acids
Theoretical weight: 91.13 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: Q05946 (Residues: 1-817; Coverage: 100%)
Gene names: UTP13, YLR222C
Sequence domains:
U3 small nucleolar RNA-associated protein 18 Chain: B8
Molecule details ›
Chain: B8
Length: 594 amino acids
Theoretical weight: 66.49 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P40362 (Residues: 1-594; Coverage: 100%)
Gene names: HRE594, J1098, UTP18, YJL069C
Sequence domains: WD domain, G-beta repeat
U3 small nucleolar RNA-associated protein 21 Chain: BE
Molecule details ›
Chain: BE
Length: 939 amino acids
Theoretical weight: 104.93 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: Q06078 (Residues: 1-939; Coverage: 100%)
Gene names: L8084.22, UTP21, YLR409C
Sequence domains:
U3 small nucleolar RNA-associated protein 6 Chain: B6
Molecule details ›
Chain: B6
Length: 440 amino acids
Theoretical weight: 52.5 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: Q02354 (Residues: 1-440; Coverage: 100%)
Gene names: D9461.34, UTP6, YDR449C
Sequence domains:
U3 small nucleolar RNA-associated protein 16 Chain: 5B
Molecule details ›
Chain: 5B
Length: 214 amino acids
Theoretical weight: 24.43 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: Q08492 (Residues: 1-214; Coverage: 100%)
Gene names: BUD21, UTP16, YOR078W, YOR29-29
Sequence domains: U3 snoRNA associated
U3 small nucleolar RNA-associated protein 7 Chain: 5C
Molecule details ›
Chain: 5C
Length: 554 amino acids
Theoretical weight: 62.42 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P40055 (Residues: 1-554; Coverage: 100%)
Gene names: KRE31, UTP7, YER082C
Sequence domains:
U3 small nucleolar RNA-associated protein 11 Chain: 5D
Molecule details ›
Chain: 5D
Length: 250 amino acids
Theoretical weight: 29.81 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P34247 (Residues: 1-250; Coverage: 100%)
Gene names: UTP11, YKL099C, YKL449
Sequence domains: Utp11 protein
U3 small nucleolar RNA-associated protein MPP10 Chain: 5E
Molecule details ›
Chain: 5E
Length: 593 amino acids
Theoretical weight: 67.04 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P47083 (Residues: 1-593; Coverage: 100%)
Gene names: J1411, MPP10, YJR002W, YJR83.5
Sequence domains: Mpp10 protein
U3 small nucleolar ribonucleoprotein protein IMP3 Chain: 5F
Molecule details ›
Chain: 5F
Length: 183 amino acids
Theoretical weight: 21.93 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P32899 (Residues: 1-183; Coverage: 100%)
Gene names: IMP3, YHR148W
Sequence domains:
U3 small nucleolar ribonucleoprotein protein IMP4 Chain: 5G
Molecule details ›
Chain: 5G
Length: 290 amino acids
Theoretical weight: 33.54 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P53941 (Residues: 1-290; Coverage: 100%)
Gene names: IMP4, N2353, YNL075W
Sequence domains: Brix domain
Something about silencing protein 10 Chain: 5H
Molecule details ›
Chain: 5H
Length: 610 amino acids
Theoretical weight: 70.36 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: Q12136 (Residues: 1-610; Coverage: 100%)
Gene names: D1545, SAS10, UTP3, YDL153C
Sequence domains:
Protein SOF1 Chain: 5I
Molecule details ›
Chain: 5I
Length: 489 amino acids
Theoretical weight: 56.89 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P33750 (Residues: 1-489; Coverage: 100%)
Gene names: L1339, SOF1, YLL011W
Sequence domains:
rRNA-processing protein FCF2 Chain: 5J
Molecule details ›
Chain: 5J
Length: 217 amino acids
Theoretical weight: 25.69 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: Q12035 (Residues: 1-217; Coverage: 100%)
Gene names: FCF2, L2128, YLR051C
Sequence domains: Fcf2 pre-rRNA processing
rRNA-processing protein FCF1 Chain: 5K
Molecule details ›
Chain: 5K
Length: 189 amino acids
Theoretical weight: 21.65 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: Q05498 (Residues: 1-189; Coverage: 100%)
Gene names: D9651.9, FCF1, YDR339C
Sequence domains: Fcf1
Ribosome biogenesis protein ENP2 Chain: RA
Molecule details ›
Chain: RA
Length: 707 amino acids
Theoretical weight: 81.86 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P48234 (Residues: 1-707; Coverage: 100%)
Gene names: ENP2, G6623, YGR145W
Sequence domains: NUC153 domain
U3 small nucleolar ribonucleoprotein protein LCP5 Chain: RB
Molecule details ›
Chain: RB
Length: 357 amino acids
Theoretical weight: 40.86 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P40079 (Residues: 1-357; Coverage: 100%)
Gene names: LCP5, SYGP-ORF43, YER127W
Sequence domains: Sas10/Utp3/C1D family
KRR1 small subunit processome component Chain: RC
Molecule details ›
Chain: RC
Length: 316 amino acids
Theoretical weight: 37.23 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P25586 (Residues: 1-316; Coverage: 100%)
Gene names: KRR1, YCL059C, YCL59C
Sequence domains:
U3 small nucleolar RNA-associated protein 22 Chain: RE
Molecule details ›
Chain: RE
Length: 1237 amino acids
Theoretical weight: 140.66 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P53254 (Residues: 1-1237; Coverage: 100%)
Gene names: UTP22, YGR090W
Sequence domains:
Ribosomal RNA-processing protein 7 Chain: RF
Molecule details ›
Chain: RF
Length: 297 amino acids
Theoretical weight: 34.53 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P25368 (Residues: 1-297; Coverage: 100%)
Gene names: RRP7, YCL031C, YCL184, YCL31C
Sequence domains:
Ribosomal RNA small subunit methyltransferase NEP1 Chains: RG, RH
Molecule details ›
Chains: RG, RH
Length: 252 amino acids
Theoretical weight: 27.94 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: Q06287 (Residues: 1-252; Coverage: 100%)
Gene names: EMG1, L9470.5, NEP1, YLR186W
Sequence domains: EMG1/NEP1 methyltransferase
Ribosome biogenesis protein UTP30 Chain: RI
Molecule details ›
Chain: RI
Length: 274 amino acids
Theoretical weight: 31.67 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P36144 (Residues: 1-274; Coverage: 100%)
Gene names: UTP30, YKR060W
Sequence domains: Ribosomal protein L1p/L10e family
Ribosome biogenesis protein BMS1 Chain: RJ
Molecule details ›
Chain: RJ
Length: 1183 amino acids
Theoretical weight: 135.79 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: Q08965 (Residues: 1-1183; Coverage: 100%)
Gene names: BMS1, YPL217C
Sequence domains:
RNA 3'-terminal phosphate cyclase-like protein Chain: RK
Molecule details ›
Chain: RK
Length: 367 amino acids
Theoretical weight: 40.22 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: Q08096 (Residues: 1-367; Coverage: 100%)
Gene names: RCL1, RTC1, YOL010W
Sequence domains:
RNA cytidine acetyltransferase Chains: RL, RM
Molecule details ›
Chains: RL, RM
Length: 1056 amino acids
Theoretical weight: 119.51 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P53914 (Residues: 1-1056; Coverage: 100%)
Gene names: KRE33, N1216, N1858, NAT10, RRA1, YNL132W
Sequence domains:
Nucleolar complex protein 14 Chain: RN
Molecule details ›
Chain: RN
Length: 810 amino acids
Theoretical weight: 94.46 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: Q99207 (Residues: 1-810; Coverage: 100%)
Gene names: D1566, NOP14, UTP2, YDL148C
Sequence domains: Nop14-like family
Nucleolar complex protein 4 Chain: RO
Molecule details ›
Chain: RO
Length: 552 amino acids
Theoretical weight: 63.71 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: Q06512 (Residues: 1-552; Coverage: 100%)
Gene names: NOC4, UTP19, YPR144C
Sequence domains: CBF/Mak21 family
U3 small nucleolar RNA-associated protein 20 Chain: RP
Molecule details ›
Chain: RP
Length: 2493 amino acids
Theoretical weight: 287.92 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P35194 (Residues: 1-2493; Coverage: 100%)
Gene names: UTP20, YBL004W, YBL0101
Sequence domains:
U3 small nucleolar RNA-associated protein 14 Chain: RQ
Molecule details ›
Chain: RQ
Length: 899 amino acids
Theoretical weight: 103.19 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: Q04500 (Residues: 1-899; Coverage: 100%)
Gene names: UTP14, YML093W
Sequence domains: Utp14 protein
Essential nuclear protein 1 Chain: RS
Molecule details ›
Chain: RS
Length: 483 amino acids
Theoretical weight: 55.21 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P38333 (Residues: 1-483; Coverage: 100%)
Gene names: ENP1, MEG1, YBR1635, YBR247C
Sequence domains: Bystin
Pno1 Chain: RT
Molecule details ›
Chain: RT
Length: 326 amino acids
Theoretical weight: 36.36 KDa
Source organism: Saccharomyces cerevisiae S288C
Protein FAF1 Chain: RV
Molecule details ›
Chain: RV
Length: 346 amino acids
Theoretical weight: 38.96 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P40546 (Residues: 1-346; Coverage: 100%)
Gene names: FAF1, YIL019W
Regulator of rDNA transcription protein 14 Chain: RW
Molecule details ›
Chain: RW
Length: 206 amino acids
Theoretical weight: 23.9 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P40470 (Residues: 1-206; Coverage: 100%)
Gene names: RRT14, YIL127C
Sequence domains: Regular of rDNA transcription protein 14
Protein BFR2 Chain: RY
Molecule details ›
Chain: RY
Length: 534 amino acids
Theoretical weight: 61.29 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: Q06631 (Residues: 1-534; Coverage: 100%)
Gene names: BFR2, YDR299W
Sequence domains:
Unassigned helices Chain: X1
Molecule details ›
Chain: X1
Length: 347 amino acids
Theoretical weight: 29.55 KDa
Source organism: Saccharomyces cerevisiae S288C
U3 snoRNA Chain: 3A
Molecule details ›
Chain: 3A
Length: 333 nucleotides
Theoretical weight: 106.5 KDa
Sequence domains: Fungal small nucleolar RNA U3
5' ETS Chain: 5A
Molecule details ›
Chain: 5A
Length: 700 nucleotides
Theoretical weight: 225.54 KDa
18S pre-rRNA Chain: SA
Molecule details ›
Chain: SA
Length: 1808 nucleotides
Theoretical weight: 582.38 KDa
Sequence domains: Eukaryotic small subunit ribosomal RNA

Ligands and Environments

3 bound ligands:
Ligand ZN 2 x ZN
Ligand GTP 1 x GTP
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.2Å
Relevant EMDB volumes: EMD-0949

Quick links

Citations

8 review citations

RNA folding and functions of RNA helicases in ribosome biogenesis.
Mitterer et al. (2022)
7 more

1 mention without citation

Additional principles that govern the release of pre-ribosomes from the nucleolus into the nucleoplasm in yeast.
LaPeruta et al. (2023)