› 6lqp

Electron Microscopy
3.2Å resolution

Cryo-EM structure of 90S small subunit preribosomes in transition states (State A)

Released:
DOI: 10.2210/pdb6lqp/pdb
PDB 6lqp coloured by chain and viewed from the front.
PDB 6lqp coloured by chain and viewed from the side.
PDB 6lqp coloured by chain and viewed from the top.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
Cryo-EM structure of 90S small ribosomal subunit precursors in transition states.
Du Y, An W, Zhu X, Sun Q, Qi J, Ye K
Science 369 1477-1481 (2020)
PMID: 32943522
Related structures: EMD-0949

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine + pseudouridine(1191) in yeast 18S rRNA = S-adenosyl-L-homocysteine + N(1)-methylpseudouridine(1191) in yeast 18S rRNA
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 73-mer (preferred)
Entry contents:
66 distinct polypeptide molecules
3 distinct RNA molecules
Macromolecules (69 distinct):
40S ribosomal protein S1-A Chain: SC
Molecule details ›
Chain: SC
Length: 255 amino acids
Theoretical weight: 28.8 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P33442 (Residues: 1-255; Coverage: 100%)
Gene names: L9753.9, PLC1, RPS10A, RPS1A, YLR441C
Sequence domains: Ribosomal S3Ae family
40S ribosomal protein S4-A Chain: SF
Molecule details ›
Chain: SF
Length: 261 amino acids
Theoretical weight: 29.47 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P0CX35 (Residues: 1-261; Coverage: 100%)
Gene names: J2186, RPS4A, RPS7B, YJR145C
Sequence domains:
40S ribosomal protein S5 Chain: SG
Molecule details ›
Chain: SG
Length: 225 amino acids
Theoretical weight: 25.07 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P26783 (Residues: 1-225; Coverage: 100%)
Gene names: J2045, RPS2, RPS5, YJR123W
Sequence domains: Ribosomal protein S7p/S5e
40S ribosomal protein S6-A Chain: SH
Molecule details ›
Chain: SH
Length: 236 amino acids
Theoretical weight: 27.05 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P0CX37 (Residues: 1-236; Coverage: 100%)
Gene names: LPG18C, RPS101, RPS10B, RPS6A, YPL090C
Sequence domains: Ribosomal protein S6e
40S ribosomal protein S7-A Chain: SI
Molecule details ›
Chain: SI
Length: 190 amino acids
Theoretical weight: 21.66 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P26786 (Residues: 1-190; Coverage: 100%)
Gene names: RPS30, RPS7A, YOR096W, YOR3177W
Sequence domains: Ribosomal protein S7e
40S ribosomal protein S8-A Chain: SJ
Molecule details ›
Chain: SJ
Length: 200 amino acids
Theoretical weight: 22.54 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P0CX39 (Residues: 1-200; Coverage: 100%)
Gene names: RPS14A, RPS8A, YBL06.05, YBL0613, YBL072C
Sequence domains: Ribosomal protein S8e
40S ribosomal protein S9-A Chain: SK
Molecule details ›
Chain: SK
Length: 197 amino acids
Theoretical weight: 22.49 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: O13516 (Residues: 1-197; Coverage: 100%)
Gene names: RPS13A, RPS9A, YPL081W, YS11A
Sequence domains:
40S ribosomal protein S11-A Chain: SM
Molecule details ›
Chain: SM
Length: 156 amino acids
Theoretical weight: 17.79 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P0CX47 (Residues: 1-156; Coverage: 100%)
Gene names: RPS11A, RPS18A, YD9813.03, YDR025W
Sequence domains:
40S ribosomal protein S12 Chain: SN
Molecule details ›
Chain: SN
Length: 143 amino acids
Theoretical weight: 15.49 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P48589 (Residues: 1-143; Coverage: 100%)
Gene names: RPS12, YOR369C
Sequence domains: Ribosomal protein L7Ae/L30e/S12e/Gadd45 family
40S ribosomal protein S13 Chain: SO
Molecule details ›
Chain: SO
Length: 151 amino acids
Theoretical weight: 17.06 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P05756 (Residues: 1-151; Coverage: 100%)
Gene names: D4252, RPS13, RPS13C, YD9609.18, YDR064W
Sequence domains:
40S ribosomal protein S14-A Chain: SP
Molecule details ›
Chain: SP
Length: 137 amino acids
Theoretical weight: 14.56 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P06367 (Residues: 1-137; Coverage: 100%)
Gene names: CRY1, RPL59, RPS14A, YCR031C, YCR31C
Sequence domains: Ribosomal protein S11
40S ribosomal protein S16-A Chain: SR
Molecule details ›
Chain: SR
Length: 143 amino acids
Theoretical weight: 15.88 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P0CX51 (Residues: 1-143; Coverage: 100%)
Gene names: RP61R, RPS16A, YM9375.12, YMR143W
Sequence domains: Ribosomal protein S9/S16
40S ribosomal protein S18-A Chain: ST
Molecule details ›
Chain: ST
Length: 146 amino acids
Theoretical weight: 17.07 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P0CX55 (Residues: 1-146; Coverage: 100%)
Gene names: D9461.35, RPS18A, YDR450W
Sequence domains: Ribosomal protein S13/S18
40S ribosomal protein S22-B Chain: SX
Molecule details ›
Chain: SX
Length: 130 amino acids
Theoretical weight: 14.65 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: Q3E7Y3 (Residues: 1-130; Coverage: 100%)
Gene names: L8039.6, RPS22B, RPS24B, YLR367W
Sequence domains: Ribosomal protein S8
40S ribosomal protein S23-A Chain: SY
Molecule details ›
Chain: SY
Length: 145 amino acids
Theoretical weight: 16.07 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P0CX29 (Residues: 1-145; Coverage: 100%)
Gene names: G6178, RPS23A, RPS28A, YGR118W
Sequence domains: Ribosomal protein S12/S23
40S ribosomal protein S24-A Chain: SZ
Molecule details ›
Chain: SZ
Length: 135 amino acids
Theoretical weight: 15.36 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P0CX31 (Residues: 1-135; Coverage: 100%)
Gene names: RPS24A, RPS24EA, YER074W
Sequence domains: Ribosomal protein S24e
40S ribosomal protein S27-A Chain: Sc
Molecule details ›
Chain: Sc
Length: 82 amino acids
Theoretical weight: 8.89 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P35997 (Residues: 1-82; Coverage: 100%)
Gene names: RPS27A, YKL156W
Sequence domains: Ribosomal protein S27
40S ribosomal protein S28-A Chain: Sd
Molecule details ›
Chain: Sd
Length: 67 amino acids
Theoretical weight: 7.61 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: Q3E7X9 (Residues: 1-67; Coverage: 100%)
Gene names: O3600, RPS28A, RPS33, RPS33A, YOR167C
Sequence domains: Ribosomal protein S28e
rRNA 2'-O-methyltransferase fibrillarin Chains: 3B, 3C
Molecule details ›
Chains: 3B, 3C
Length: 327 amino acids
Theoretical weight: 34.53 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P15646 (Residues: 1-327; Coverage: 100%)
Gene names: D2870, LOT3, NOP1, YDL014W
Sequence domains: Fibrillarin
Nucleolar protein 56 Chain: 3D
Molecule details ›
Chain: 3D
Length: 504 amino acids
Theoretical weight: 56.96 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: Q12460 (Residues: 1-504; Coverage: 100%)
Gene names: L8167.9, NOP56, SIK1, YLR197W
Sequence domains:
Nucleolar protein 58 Chain: 3E
Molecule details ›
Chain: 3E
Length: 511 amino acids
Theoretical weight: 57.06 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: Q12499 (Residues: 1-511; Coverage: 100%)
Gene names: NOP5, NOP58, O6108, YOR310C
Sequence domains:
Ribosomal RNA-processing protein 9 Chain: 3F
Molecule details ›
Chain: 3F
Length: 573 amino acids
Theoretical weight: 65.15 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: Q06506 (Residues: 1-573; Coverage: 100%)
Gene names: RRP9, YPR137W
Sequence domains: WD domain, G-beta repeat
13 kDa ribonucleoprotein-associated protein Chains: 3G, 3H
Molecule details ›
Chains: 3G, 3H
Length: 126 amino acids
Theoretical weight: 13.58 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P39990 (Residues: 1-126; Coverage: 100%)
Gene names: SNU13, YEL026W
Sequence domains: Ribosomal protein L7Ae/L30e/S12e/Gadd45 family
U3 small nucleolar RNA-associated protein 4 Chain: A4
Molecule details ›
Chain: A4
Length: 776 amino acids
Theoretical weight: 87.91 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: Q06679 (Residues: 1-776; Coverage: 100%)
Gene names: UTP4, YDR324C
Sequence domains: WD domain, G-beta repeat
U3 small nucleolar RNA-associated protein 5 Chain: A5
Molecule details ›
Chain: A5
Length: 643 amino acids
Theoretical weight: 72.08 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: Q04177 (Residues: 1-643; Coverage: 100%)
Gene names: D9509.17, UTP5, YDR398W
Sequence domains: Dip2/Utp12 Family
U3 small nucleolar RNA-associated protein 8 Chain: A8
Molecule details ›
Chain: A8
Length: 713 amino acids
Theoretical weight: 80.27 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P53276 (Residues: 1-713; Coverage: 100%)
Gene names: UTP8, YGR128C
Sequence domains: Utp8 family
U3 small nucleolar RNA-associated protein 9 Chain: A9
Molecule details ›
Chain: A9
Length: 575 amino acids
Theoretical weight: 65.35 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P38882 (Residues: 1-575; Coverage: 100%)
Gene names: UTP9, YHR196W
U3 small nucleolar RNA-associated protein 10 Chain: AE
Molecule details ›
Chain: AE
Length: 1769 amino acids
Theoretical weight: 200.3 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P42945 (Residues: 1-1769; Coverage: 100%)
Gene names: J0808, UTP10, YJL109C
Sequence domains:
U3 small nucleolar RNA-associated protein 15 Chain: AF
Molecule details ›
Chain: AF
Length: 513 amino acids
Theoretical weight: 57.77 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: Q04305 (Residues: 1-513; Coverage: 100%)
Gene names: UTP15, YM9582.18, YMR093W
Sequence domains:
NET1-associated nuclear protein 1 Chain: AG
Molecule details ›
Chain: AG
Length: 896 amino acids
Theoretical weight: 101.34 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: Q02931 (Residues: 1-896; Coverage: 100%)
Gene names: NAN1, UTP17, YPL126W
Sequence domains: WD domain, G-beta repeat
Periodic tryptophan protein 2 Chain: B1
Molecule details ›
Chain: B1
Length: 923 amino acids
Theoretical weight: 104.1 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P25635 (Residues: 1-923; Coverage: 100%)
Gene names: PWP2, UTP1, YCR057C, YCR55C/YCR57C/YCR58C
Sequence domains:
U3 small nucleolar RNA-associated protein 12 Chain: B2
Molecule details ›
Chain: B2
Length: 943 amino acids
Theoretical weight: 106.48 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: Q12220 (Residues: 1-943; Coverage: 100%)
Gene names: DIP2, L3116, L9233.1, UTP12, YLR129W
Sequence domains:
U3 small nucleolar RNA-associated protein 13 Chain: B3
Molecule details ›
Chain: B3
Length: 817 amino acids
Theoretical weight: 91.13 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: Q05946 (Residues: 1-817; Coverage: 100%)
Gene names: UTP13, YLR222C
Sequence domains:
U3 small nucleolar RNA-associated protein 18 Chain: B8
Molecule details ›
Chain: B8
Length: 594 amino acids
Theoretical weight: 66.49 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P40362 (Residues: 1-594; Coverage: 100%)
Gene names: HRE594, J1098, UTP18, YJL069C
Sequence domains: WD domain, G-beta repeat
U3 small nucleolar RNA-associated protein 21 Chain: BE
Molecule details ›
Chain: BE
Length: 939 amino acids
Theoretical weight: 104.93 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: Q06078 (Residues: 1-939; Coverage: 100%)
Gene names: L8084.22, UTP21, YLR409C
Sequence domains:
U3 small nucleolar RNA-associated protein 6 Chain: B6
Molecule details ›
Chain: B6
Length: 440 amino acids
Theoretical weight: 52.5 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: Q02354 (Residues: 1-440; Coverage: 100%)
Gene names: D9461.34, UTP6, YDR449C
Sequence domains: U3 small nucleolar RNA-associated protein 6
Bud site selection protein 21 Chain: 5B
Molecule details ›
Chain: 5B
Length: 214 amino acids
Theoretical weight: 24.43 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: Q08492 (Residues: 1-214; Coverage: 100%)
Gene names: BUD21, UTP16, YOR078W, YOR29-29
Sequence domains: U3 snoRNA associated
U3 small nucleolar RNA-associated protein 7 Chain: 5C
Molecule details ›
Chain: 5C
Length: 554 amino acids
Theoretical weight: 62.42 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P40055 (Residues: 1-554; Coverage: 100%)
Gene names: KRE31, UTP7, YER082C
Sequence domains: BING4CT (NUC141) domain
U3 small nucleolar RNA-associated protein 11 Chain: 5D
Molecule details ›
Chain: 5D
Length: 250 amino acids
Theoretical weight: 29.81 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P34247 (Residues: 1-250; Coverage: 100%)
Gene names: UTP11, YKL099C, YKL449
Sequence domains: Utp11 protein
U3 small nucleolar RNA-associated protein MPP10 Chain: 5E
Molecule details ›
Chain: 5E
Length: 593 amino acids
Theoretical weight: 67.04 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P47083 (Residues: 1-593; Coverage: 100%)
Gene names: J1411, MPP10, YJR002W, YJR83.5
Sequence domains: Mpp10 protein
U3 small nucleolar ribonucleoprotein protein IMP3 Chain: 5F
Molecule details ›
Chain: 5F
Length: 183 amino acids
Theoretical weight: 21.93 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P32899 (Residues: 1-183; Coverage: 100%)
Gene names: IMP3, YHR148W
Sequence domains:
U3 small nucleolar ribonucleoprotein protein IMP4 Chain: 5G
Molecule details ›
Chain: 5G
Length: 290 amino acids
Theoretical weight: 33.54 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P53941 (Residues: 1-290; Coverage: 100%)
Gene names: IMP4, N2353, YNL075W
Sequence domains: Brix domain
Something about silencing protein 10 Chain: 5H
Molecule details ›
Chain: 5H
Length: 610 amino acids
Theoretical weight: 70.36 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: Q12136 (Residues: 1-610; Coverage: 100%)
Gene names: D1545, SAS10, UTP3, YDL153C
Sequence domains: Sas10 C-terminal domain
Protein SOF1 Chain: 5I
Molecule details ›
Chain: 5I
Length: 489 amino acids
Theoretical weight: 56.89 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P33750 (Residues: 1-489; Coverage: 100%)
Gene names: L1339, SOF1, YLL011W
Sequence domains:
rRNA-processing protein FCF2 Chain: 5J
Molecule details ›
Chain: 5J
Length: 217 amino acids
Theoretical weight: 25.69 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: Q12035 (Residues: 1-217; Coverage: 100%)
Gene names: FCF2, L2128, YLR051C
Sequence domains: Fcf2 pre-rRNA processing
rRNA-processing protein FCF1 Chain: 5K
Molecule details ›
Chain: 5K
Length: 189 amino acids
Theoretical weight: 21.65 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: Q05498 (Residues: 1-189; Coverage: 100%)
Gene names: D9651.9, FCF1, YDR339C
Sequence domains: Fcf1
Ribosome biogenesis protein ENP2 Chain: RA
Molecule details ›
Chain: RA
Length: 707 amino acids
Theoretical weight: 81.86 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P48234 (Residues: 1-707; Coverage: 100%)
Gene names: ENP2, G6623, YGR145W
Sequence domains: NUC153 domain
U3 small nucleolar ribonucleoprotein protein LCP5 Chain: RB
Molecule details ›
Chain: RB
Length: 357 amino acids
Theoretical weight: 40.86 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P40079 (Residues: 1-357; Coverage: 100%)
Gene names: LCP5, SYGP-ORF43, YER127W
Sequence domains: Sas10/Utp3/C1D family
KRR1 small subunit processome component Chain: RC
Molecule details ›
Chain: RC
Length: 316 amino acids
Theoretical weight: 37.23 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P25586 (Residues: 1-316; Coverage: 100%)
Gene names: KRR1, YCL059C, YCL59C
Sequence domains: Krr1 KH1 domain
U3 small nucleolar RNA-associated protein 22 Chain: RE
Molecule details ›
Chain: RE
Length: 1237 amino acids
Theoretical weight: 140.66 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P53254 (Residues: 1-1237; Coverage: 100%)
Gene names: UTP22, YGR090W
Sequence domains:
Ribosomal RNA-processing protein 7 Chain: RF
Molecule details ›
Chain: RF
Length: 297 amino acids
Theoretical weight: 34.53 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P25368 (Residues: 1-297; Coverage: 100%)
Gene names: RRP7, YCL031C, YCL184, YCL31C
Sequence domains:
Ribosomal RNA small subunit methyltransferase NEP1 Chains: RG, RH
Molecule details ›
Chains: RG, RH
Length: 252 amino acids
Theoretical weight: 27.94 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: Q06287 (Residues: 1-252; Coverage: 100%)
Gene names: EMG1, L9470.5, NEP1, YLR186W
Sequence domains: EMG1/NEP1 methyltransferase
Ribosome biogenesis protein UTP30 Chain: RI
Molecule details ›
Chain: RI
Length: 274 amino acids
Theoretical weight: 31.67 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P36144 (Residues: 1-274; Coverage: 100%)
Gene names: UTP30, YKR060W
Sequence domains: Ribosomal protein L1p/L10e family
Ribosome biogenesis protein BMS1 Chain: RJ
Molecule details ›
Chain: RJ
Length: 1183 amino acids
Theoretical weight: 135.79 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: Q08965 (Residues: 1-1183; Coverage: 100%)
Gene names: BMS1, YPL217C
Sequence domains:
RNA 3'-terminal phosphate cyclase-like protein Chain: RK
Molecule details ›
Chain: RK
Length: 367 amino acids
Theoretical weight: 40.22 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: Q08096 (Residues: 1-367; Coverage: 100%)
Gene names: RCL1, RTC1, YOL010W
Sequence domains:
RNA cytidine acetyltransferase Chains: RL, RM
Molecule details ›
Chains: RL, RM
Length: 1056 amino acids
Theoretical weight: 119.51 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P53914 (Residues: 1-1056; Coverage: 100%)
Gene names: KRE33, N1216, N1858, NAT10, RRA1, YNL132W
Sequence domains:
Nucleolar complex protein 14 Chain: RN
Molecule details ›
Chain: RN
Length: 810 amino acids
Theoretical weight: 94.46 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: Q99207 (Residues: 1-810; Coverage: 100%)
Gene names: D1566, NOP14, UTP2, YDL148C
Sequence domains: Nop14-like family
Nucleolar complex protein 4 Chain: RO
Molecule details ›
Chain: RO
Length: 552 amino acids
Theoretical weight: 63.71 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: Q06512 (Residues: 1-552; Coverage: 100%)
Gene names: NOC4, UTP19, YPR144C
Sequence domains: CBF/Mak21 family
U3 small nucleolar RNA-associated protein 20 Chain: RP
Molecule details ›
Chain: RP
Length: 2493 amino acids
Theoretical weight: 287.92 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P35194 (Residues: 1-2493; Coverage: 100%)
Gene names: UTP20, YBL004W, YBL0101
Sequence domains:
U3 small nucleolar RNA-associated protein 14 Chain: RQ
Molecule details ›
Chain: RQ
Length: 899 amino acids
Theoretical weight: 103.19 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: Q04500 (Residues: 1-899; Coverage: 100%)
Gene names: UTP14, YML093W
Sequence domains: Utp14 protein
Essential nuclear protein 1 Chain: RS
Molecule details ›
Chain: RS
Length: 483 amino acids
Theoretical weight: 55.21 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P38333 (Residues: 1-483; Coverage: 100%)
Gene names: ENP1, MEG1, YBR1635, YBR247C
Sequence domains: Bystin
Pno1 Chain: RT
Molecule details ›
Chain: RT
Length: 326 amino acids
Theoretical weight: 36.36 KDa
Source organism: Saccharomyces cerevisiae S288C
Protein FAF1 Chain: RV
Molecule details ›
Chain: RV
Length: 346 amino acids
Theoretical weight: 38.96 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P40546 (Residues: 1-346; Coverage: 100%)
Gene names: FAF1, YIL019W
Regulator of rDNA transcription protein 14 Chain: RW
Molecule details ›
Chain: RW
Length: 206 amino acids
Theoretical weight: 23.9 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P40470 (Residues: 1-206; Coverage: 100%)
Gene names: RRT14, YIL127C
Sequence domains: Regular of rDNA transcription protein 14
Protein BFR2 Chain: RY
Molecule details ›
Chain: RY
Length: 534 amino acids
Theoretical weight: 61.29 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: Q06631 (Residues: 1-534; Coverage: 100%)
Gene names: BFR2, YDR299W
Sequence domains:
Unassigned helices Chain: X1
Molecule details ›
Chain: X1
Length: 347 amino acids
Theoretical weight: 29.55 KDa
Source organism: Saccharomyces cerevisiae S288C
U3 snoRNA Chain: 3A
Molecule details ›
Chain: 3A
Length: 333 nucleotides
Theoretical weight: 106.5 KDa
Sequence domains: Fungal small nucleolar RNA U3
5' ETS Chain: 5A
Molecule details ›
Chain: 5A
Length: 700 nucleotides
Theoretical weight: 225.54 KDa
18S pre-rRNA Chain: SA
Molecule details ›
Chain: SA
Length: 1808 nucleotides
Theoretical weight: 582.38 KDa
Sequence domains: Eukaryotic small subunit ribosomal RNA

Ligands and Environments

3 bound ligands:
Ligand ZN 2 x ZN
Ligand GTP 1 x GTP
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.2Å
Relevant EMDB volumes: EMD-0949

Quick links

Citations

1 citations in other articles

Bud23 promotes the final disassembly of the small subunit Processome in Saccharomyces cerevisiae.
Black et al. (2020)