X-ray diffraction
2.49Å resolution

The crystal structure of human cytoplasmic LRS


Function and Biology Details

Reaction catalysed:
ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu)
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Leucine--tRNA ligase, cytoplasmic Chains: A, B
Molecule details ›
Chains: A, B
Length: 1092 amino acids
Theoretical weight: 125.19 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
  • Canonical: Q9P2J5 (Residues: 1-1070; Coverage: 91%)
Gene names: KIAA1352, LARS, LARS1
Sequence domains:

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: C2221
Unit cell:
a: 88.217Å b: 94.684Å c: 680.055Å
α: 90° β: 90° γ: 90°
R R work R free
0.201 0.198 0.242
Expression system: Escherichia coli