6lh1

X-ray diffraction
2.86Å resolution

Crystal structure of a cysteine-pair mutant (Y113C-P190C) of a bacterial bile acid transporter trapped in an outward-facing conformation

Released:
Source organism: Yersinia frederiksenii
Primary publication:
An engineered disulfide bridge traps and validates an outward-facing conformation in a bile acid transporter.
Acta Crystallogr D Struct Biol 77 108-116 (2021)
PMID: 33404530

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Transporter, sodium/bile acid symporter family Chain: A
Molecule details ›
Chain: A
Length: 312 amino acids
Theoretical weight: 33.31 KDa
Source organism: Yersinia frederiksenii
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A380PV03 (Residues: 1-307; Coverage: 100%)
Gene name: NCTC11470_02445
Sequence domains: Sodium Bile acid symporter family

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: I222
Unit cell:
a: 43.366Å b: 77.005Å c: 193.114Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.23 0.229 0.264
Expression system: Escherichia coli