› 6lfd

X-ray diffraction
1.92Å resolution

Crystal structure of VMB-1 at pH5.5(Bis-Tris)

Released:
DOI: 10.2210/pdb6lfd/pdb
PDB entry 6lfd coloured by chain, front view.
PDB entry 6lfd coloured by chain, side view.
PDB entry 6lfd coloured by chain, top view.
Source organism: Vibrio alginolyticus
Entry authors: Cheng Q, Chen S

Function and Biology Details

Reaction catalysed: 
A beta-lactam + H(2)O = a substituted beta-amino acid
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-105481 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Metallo-beta-lactamase type 2 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 234 amino acids
Theoretical weight: 25.71 KDa
Source organism: Vibrio alginolyticus
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: A0A5Q5ADH9 (Residues: 22-251; Coverage: 92%)
Gene name: vmb-1
Sequence domains: Metallo-beta-lactamase superfamily

Ligands and Environments

2 bound ligands:
Ligand ZN 8 x ZN
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U1
Spacegroup: P212121
Unit cell:
a: 73.11Å b: 97.11Å c: 132.66Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.202 0.2 0.239
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

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