› 6ley

X-ray diffraction
2.39Å resolution

Structure of Sil1G bound FEM1C

Released:
DOI: 10.2210/pdb6ley/pdb
PDB entry 6ley coloured by chain, front view.
PDB entry 6ley coloured by chain, side view.
PDB entry 6ley coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Molecular basis for arginine C-terminal degron recognition by Cul2FEM1 E3 ligase.
Chen X, Liao S, Makaros Y, Guo Q, Zhu Z, Krizelman R, Dahan K, Tu X, Yao X, Koren I, Xu C
Nat Chem Biol 17 254-262 (2021)
PMID: 33398168

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188584 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nucleotide exchange factor SIL1; Protein fem-1 homolog C Chains: A, B
Molecule details ›
Chains: A, B
Length: 419 amino acids
Theoretical weight: 45.62 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q96JP0 (Residues: 1-390; Coverage: 63%)
  • Canonical: Q9H173 (Residues: 452-461; Coverage: 2%)
Gene names: FEM1C, KIAA1785, SIL1, UNQ545/PRO836
Sequence domains: Ankyrin repeats (3 copies)

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U1
Spacegroup: P212121
Unit cell:
a: 95.497Å b: 95.973Å c: 147.478Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.199 0.198 0.229
Expression system: Escherichia coli

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Citations

2 review citations

How the ends signal the end: Regulation by E3 ubiquitin ligases recognizing protein termini.
Sherpa et al. (2022)
1 more