› 6kvc

X-ray diffraction
1.66Å resolution

MoeE5 in complex with UDP-glucose and NAD

Released:
DOI: 10.2210/pdb6kvc/pdb
PDB entry 6kvc coloured by chain, front view.
PDB entry 6kvc coloured by chain, side view.
PDB entry 6kvc coloured by chain, top view.
Source organism: Streptomyces viridosporus ATCC 14672
Primary publication:
Structure of an antibiotic-synthesizing UDP-glucuronate 4-epimerase MoeE5 in complex with substrate.
Sun H, Ko TP, Liu W, Liu W, Zheng Y, Chen CC, Guo RT
Biochem Biophys Res Commun 521 31-36 (2020)
PMID: 31653344

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-100001 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NAD-dependent epimerase/dehydratase domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 346 amino acids
Theoretical weight: 36.54 KDa
Source organism: Streptomyces viridosporus ATCC 14672
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: A0A003 (Residues: 1-340; Coverage: 100%)
Gene name: moeE5
Sequence domains: NAD dependent epimerase/dehydratase family

Ligands and Environments

2 bound ligands:
Ligand NAD 1 x NAD
Ligand UPG 1 x UPG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL15A1
Spacegroup: P41212
Unit cell:
a: 59.047Å b: 59.047Å c: 197.975Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.156 0.155 0.191
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

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Citations

3 citation in other articles

Mechanistic characterization of UDP-glucuronic acid 4-epimerase.
Borg et al. (2021)
2 more