6knc

Electron Microscopy
9.3Å resolution

Function and Biology Details

Reactions catalysed:
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Exonucleolytic cleavage in the 3'- to 5'-direction to yield nucleoside 5'-phosphates
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero heptamer (preferred)
Entry contents:
3 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (5 distinct):
DNA polymerase II small subunit Chain: A
Molecule details ›
Chain: A
Length: 537 amino acids
Theoretical weight: 60.77 KDa
Source organism: Thermococcus kodakarensis KOD1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5JET1 (Residues: 259-735; Coverage: 65%)
Gene names: TK1902, polB
Sequence domains: DNA polymerase alpha/epsilon subunit B
DNA polymerase II large subunit, 1st part Chain: B
Molecule details ›
Chain: B
Length: 1324 amino acids
Theoretical weight: 150.42 KDa
Source organism: Thermococcus kodakarensis
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5JET0 (Residues: 1-1798; Coverage: 74%)
Gene names: TK1903, polC
Sequence domains: DNA polymerase II large subunit DP2
DNA polymerase sliding clamp 1 Chains: C, D, E
Molecule details ›
Chains: C, D, E
Length: 249 amino acids
Theoretical weight: 28.27 KDa
Source organism: Thermococcus kodakarensis KOD1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5JF32 (Residues: 1-249; Coverage: 100%)
Gene names: TK0535, pcn1
Sequence domains:
pri30DNA Chain: F
Molecule details ›
Chain: F
Length: 30 nucleotides
Theoretical weight: 9.23 KDa
temp45DNA Chain: G
Molecule details ›
Chain: G
Length: 30 nucleotides
Theoretical weight: 9.21 KDa

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 9.3Å
Relevant EMDB volumes: EMD-0725
Expression system: Escherichia coli