6klb

Electron Microscopy
4.1Å resolution

Structure of LbCas12a-crRNA complex bound to AcrVA4 (form B complex)

Released:
Primary publication:
Structural insight into multistage inhibition of CRISPR-Cas12a by AcrVA4.
Proc. Natl. Acad. Sci. U.S.A. (2019)
PMID: 31467167
Related structures: EMD-0705

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Entry contents:
2 distinct polypeptide molecules
1 distinct RNA molecule
Macromolecules (3 distinct):
LbCas12a Chains: A, D
Molecule details ›
Chains: A, D
Length: 1228 amino acids
Theoretical weight: 143.89 KDa
Source organism: Lachnospiraceae bacterium
Expression system: Escherichia coli BL21
AcrVA4 Chains: B, C
Molecule details ›
Chains: B, C
Length: 234 amino acids
Theoretical weight: 27.37 KDa
Source organism: Moraxella bovoculi
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: A0A0U2APF4 (Residues: 1-234; Coverage: 100%)
Gene name: AAX07_09545
crRNA Chains: E, G
Molecule details ›
Chains: E, G
Length: 42 nucleotides
Theoretical weight: 13.48 KDa

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.1Å
Relevant EMDB volumes: EMD-0705
Expression system: Escherichia coli BL21