X-ray diffraction
3.5Å resolution

Crystal structure of Drug:Proton Antiporter-1 (DHA1) Family SotB, in the inward open conformation (H115A mutant)

Source organism: Escherichia coli K-12
Primary publication:
Visualizing the nonlinear changes of a drug-proton antiporter from inward-open to occluded state.
Biochem Biophys Res Commun 534 272-278 (2021)
PMID: 33280821

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Sugar efflux transporter Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 423 amino acids
Theoretical weight: 45.42 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
  • Canonical: P31122 (Residues: 1-396; Coverage: 100%)
Gene names: JW1521, b1528, sotB, ydeA
Sequence domains: Major Facilitator Superfamily

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: C2
Unit cell:
a: 261.618Å b: 61.822Å c: 122.463Å
α: 90° β: 93.42° γ: 90°
R R work R free
0.234 0.231 0.267
Expression system: Escherichia coli BL21(DE3)