› 6kke

X-ray diffraction
2.58Å resolution

THRb mutation with a novel agonist

Released:
DOI: 10.2210/pdb6kke/pdb
PDB entry 6kke coloured by chain, front view.
PDB entry 6kke coloured by chain, side view.
PDB entry 6kke coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Revealing a Mutant-Induced Receptor Allosteric Mechanism for the Thyroid Hormone Resistance.
Yao B, Wei Y, Zhang S, Tian S, Xu S, Wang R, Zheng W, Li Y
iScience 20 489-496 (2019)
PMID: 31655060

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145483 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Thyroid hormone receptor beta Chain: A
Molecule details ›
Chain: A
Length: 249 amino acids
Theoretical weight: 28.12 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P10828 (Residues: 211-459; Coverage: 54%)
Gene names: ERBA2, NR1A2, THR1, THRB
Sequence domains: Ligand-binding domain of nuclear hormone receptor
Nuclear receptor coactivator 2 Chain: C
Molecule details ›
Chain: C
Length: 11 amino acids
Theoretical weight: 1.35 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q15596 (Residues: 741-751; Coverage: 1%)
Gene names: BHLHE75, NCOA2, SRC2, TIF2

Ligands and Environments

1 bound ligand:
Ligand 8HO 1 x 8HO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL18U1
Spacegroup: P43212
Unit cell:
a: 106.732Å b: 106.732Å c: 57.079Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.186 0.184 0.217
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 review citations

Resistance to Thyroid Hormone Beta: A Focused Review.
Pappa et al. (2021)
4 more