› 6khz

X-ray diffraction
2.8Å resolution

p62/SQSTM1 ZZ domain with Gly-peptide

Released:
DOI: 10.2210/pdb6khz/pdb
PDB entry 6khz coloured by chain, front view.
PDB entry 6khz coloured by chain, side view.
PDB entry 6khz coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Use of the LC3B-fusion technique for biochemical and structural studies of proteins involved in the N-degron pathway.
Kim L, Kwon DH, Heo J, Park MR, Song HK
J Biol Chem 295 2590-2600 (2020)
PMID: 31919097

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171721 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sequestosome-1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 50 amino acids
Theoretical weight: 5.41 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q13501 (Residues: 125-169; Coverage: 10%)
Gene names: ORCA, OSIL, SQSTM1
Sequence domains: Zinc finger, ZZ type

Ligands and Environments

1 bound ligand:
Ligand ZN 8 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: I23
Unit cell:
a: 113.979Å b: 113.979Å c: 113.979Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.24 0.235 0.287
Expression system: Escherichia coli

Quick links

Citations

2 review citations

How the ends signal the end: Regulation by E3 ubiquitin ligases recognizing protein termini.
Sherpa et al. (2022)
1 more

3 mentions without citation

Behavioural and neurobiological implications of linear and non-linear features in larynx phonations of horseshoe bats.
Kobayasi et al. (2012)
2 more