6kbx

X-ray diffraction
1.22Å resolution

Crystal structure of yedK in complex with ssDNA containing abasic site

Released:
Source organism: Escherichia coli

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-120129 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Abasic site processing protein YedK Chain: B
Molecule details ›
Chain: B
Length: 227 amino acids
Theoretical weight: 25.61 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: P76318 (Residues: 2-222; Coverage: 100%)
Gene names: JW1916, b1931, yedG, yedK
Sequence domains: SOS response associated peptidase (SRAP)
DNA 5'-D (*CP*GP*GP*TP* (PED)P*GP*AP*TP*TP*C)-3' Chain: A
Molecule details ›
Chain: A
Length: 10 nucleotides
Theoretical weight: 2.93 KDa
Source organism: Escherichia coli
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: P21
Unit cell:
a: 47.092Å b: 44.084Å c: 54.731Å
α: 90° β: 101.69° γ: 90°
R-values:
R R work R free
0.156 0.155 0.165
Expression systems:
  • Escherichia coli 'BL21-Gold(DE3)pLysS AG'
  • Not provided