› 6k7f

X-ray diffraction
1.8Å resolution

Crystal structure of MBPholo-Tim21 fusion protein with a 17-residue helical linker

Released:
DOI: 10.2210/pdb6k7f/pdb
PDB entry 6k7f coloured by chain, front view.
PDB entry 6k7f coloured by chain, side view.
PDB entry 6k7f coloured by chain, top view.
Source organisms:
Primary publication:
Crystal contact-free conformation of an intrinsically flexible loop in protein crystal: Tim21 as the case study.
Bala S, Shinya S, Srivastava A, Ishikawa M, Shimada A, Kobayashi N, Kojima C, Tama F, Miyashita O, Kohda D
Biochim Biophys Acta Gen Subj 1864 129418 (2020)
PMID: 31449839

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142542 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Maltose/maltodextrin-binding periplasmic protein; Mitochondrial import inner membrane translocase subunit TIM21 Chain: A
Molecule details ›
Chain: A
Length: 499 amino acids
Theoretical weight: 55.65 KDa
Source organisms: Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0AEX9 (Residues: 27-394; Coverage: 100%)
  • Canonical: P53220 (Residues: 105-217; Coverage: 47%)
Gene names: JW3994, TIM21, YGR033C, b4034, malE
Sequence domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC
Carbohydrate polymer
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44XU
Spacegroup: P212121
Unit cell:
a: 40.97Å b: 69.41Å c: 171.36Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.177 0.174 0.216
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 citation in other articles

Simple Selection Procedure to Distinguish between Static and Flexible Loops.
Mitusińska et al. (2020)
1 more