› 6k6a

X-ray diffraction
1.94Å resolution

Application of anti-helix antibodies in protein structure determination (8188cys-3LRHcys)

Released:
DOI: 10.2210/pdb6k6a/pdb
PDB entry 6k6a coloured by chain, front view.
PDB entry 6k6a coloured by chain, side view.
PDB entry 6k6a coloured by chain, top view.
Source organisms:
Primary publication:
Application of antihelix antibodies in protein structure determination.
Kim JW, Kim S, Lee H, Cho G, Kim SC, Lee H, Jin MS, Lee JO
Proc Natl Acad Sci U S A 116 17786-17791 (2019)
PMID: 31371498

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-208868 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
3LRH intrabody Chains: A, B
Molecule details ›
Chains: A, B
Length: 135 amino acids
Theoretical weight: 14.14 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Immunoglobulin G-binding protein A Chains: C, D
Molecule details ›
Chains: C, D
Length: 79 amino acids
Theoretical weight: 8.89 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli
UniProt:
  • Canonical: P02976 (Residues: 100-151; Coverage: 11%)
Gene names: SAOUHSC_00069, spa
Sequence domains: B domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 7A (6B, 6C1)
Spacegroup: P1
Unit cell:
a: 37.227Å b: 40.603Å c: 61.691Å
α: 87.16° β: 75.2° γ: 65.5°
R-values:
R R work R free
0.174 0.169 0.215
Expression system: Escherichia coli

Quick links

Citations

8 citation in other articles

Synthetic antibodies against BRIL as universal fiducial marks for single-particle cryoEM structure determination of membrane proteins.
Mukherjee et al. (2020)
7 more

2 mentions without citation

Application of antihelix antibodies in protein structure determination.
Kim et al. (2019)
1 more