› 6k06

X-ray diffraction
1.75Å resolution

Crystal structure of Importin-alpha and phosphomimetic GM130

Released:
DOI: 10.2210/pdb6k06/pdb
PDB entry 6k06 coloured by chain, front view.
PDB entry 6k06 coloured by chain, side view.
PDB entry 6k06 coloured by chain, top view.
Source organisms:
Primary publication:
Ran pathway-independent regulation of mitotic Golgi disassembly by Importin-α.
Chang CC, Chen CJ, Grauffel C, Pien YC, Lim C, Tsai SY, Hsia KC
Nat Commun 10 4307 (2019)
PMID: 31541088

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156518 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Golgin subfamily A member 2 Chain: A
Molecule details ›
Chain: A
Length: 53 amino acids
Theoretical weight: 5.8 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q08379 (Residues: 13-60; Coverage: 5%)
Gene name: GOLGA2
Importin subunit alpha-1 Chain: C
Molecule details ›
Chain: C
Length: 433 amino acids
Theoretical weight: 46.9 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P52293 (Residues: 70-498; Coverage: 81%)
Gene names: Kpna2, Rch1
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE TPS 05A
Spacegroup: P212121
Unit cell:
a: 73.734Å b: 79.001Å c: 89.569Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.172 0.172 0.199
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Karyopherin-mediated nucleocytoplasmic transport.
Wing et al. (2022)
6 more

1 mention without citation

Mechanistic insights into the inhibitory activity of FDA approved ivermectin against SARS-CoV-2: old drug with new implications.
Qureshi et al. (2022)