› 6jvu

X-ray diffraction
2.8Å resolution

Crystal structure of Klebsiella pneumoniae CysE in complex with L-cysteine

Released:
DOI: 10.2210/pdb6jvu/pdb
PDB entry 6jvu coloured by chain, front view.
PDB entry 6jvu coloured by chain, side view.
PDB entry 6jvu coloured by chain, top view.
Source organism: Klebsiella pneumoniae MGH 78578
Primary publication:
Allosteric inhibition and kinetic characterization of Klebsiella pneumoniae CysE: An emerging drug target.
Verma D, Gupta S, Saxena R, Kaur P, R R, Srivastava S, Gupta V
Int J Biol Macromol 151 1240-1249 (2020)
PMID: 31751684

Function and Biology Details

Reaction catalysed: 
Acetyl-CoA + L-serine = CoA + O-acetyl-L-serine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-108091 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Serine acetyltransferase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 281 amino acids
Theoretical weight: 30.33 KDa
Source organism: Klebsiella pneumoniae MGH 78578
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A6TFK2 (Residues: 1-273; Coverage: 100%)
Gene names: KPN_03951, cysE
Sequence domains:

Ligands and Environments

3 bound ligands:
Ligand EDO 1 x EDO
Ligand CYS 6 x CYS
Ligand PO4 2 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: P21
Unit cell:
a: 68.77Å b: 110.75Å c: 101.79Å
α: 90° β: 96.62° γ: 90°
R-values:
R R work R free
0.224 0.223 0.257
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Combatting antimicrobial resistance via the cysteine biosynthesis pathway in bacterial pathogens.
Hicks et al. (2022)
1 other article

2 mentions without citation

New insights into the structure and function of an emerging drug target CysE.
Verma et al. (2021)
1 more