6jp6

X-ray diffraction
2.7Å resolution

The X-ray structure of yeast tRNA methyltransferase complex of Trm7 and Trm734 essential for 2'-O-methylation at the first position of anticodon in specific tRNAs

Released:

Function and Biology Details

Reaction catalysed:
S-adenosyl-L-methionine + cytidine(32)/guanosine(34) in tRNA = S-adenosyl-L-homocysteine + 2'-O-methylcytidine(32)/2'-O-methylguanosine(34) in tRNA
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Regulator of Ty1 transposition protein 10 Chains: A, C
Molecule details ›
Chains: A, C
Length: 1028 amino acids
Theoretical weight: 116.39 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: Q08924 (Residues: 1-1013; Coverage: 100%)
Gene names: ERE2, RTT10, TRM734, YPL183C
Sequence domains: WD domain, G-beta repeat
tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase Chains: B, D
Molecule details ›
Chains: B, D
Length: 325 amino acids
Theoretical weight: 36.55 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P38238 (Residues: 1-310; Coverage: 100%)
Gene names: TRM7, YBR0527, YBR061C
Sequence domains: FtsJ-like methyltransferase

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL38B1
Spacegroup: C2
Unit cell:
a: 253.643Å b: 110.351Å c: 133.503Å
α: 90° β: 90.02° γ: 90°
R-values:
R R work R free
0.196 0.194 0.239
Expression system: Escherichia coli