› 6jix

X-ray diffraction
2.65Å resolution

The cyrstal structure of taurine:2-oxoglutarate aminotransferase from Bifidobacterium kashiwanohense, in complex with PLP and glutamate

Released:
DOI: 10.2210/pdb6jix/pdb
PDB entry 6jix coloured by chain, front view.
PDB entry 6jix coloured by chain, side view.
PDB entry 6jix coloured by chain, top view.
Source organism: Bifidobacterium kashiwanohense PV20-2
Primary publication:
Biochemical and structural investigation of taurine:2-oxoglutarate aminotransferase from Bifidobacterium kashiwanohense.
Li M, Wei Y, Yin J, Lin L, Zhou Y, Hua G, Cao P, Ang EL, Zhao H, Yuchi Z, Zhang Y
Biochem J 476 1605-1619 (2019)
PMID: 31088892

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-100840 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Adenosylmethionine-8-amino-7-oxononanoate aminotransferase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 449 amino acids
Theoretical weight: 49.85 KDa
Source organism: Bifidobacterium kashiwanohense PV20-2
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: A0A0A7I435 (Residues: 1-447; Coverage: 100%)
Gene name: AH68_00255
Sequence domains: Aminotransferase class-III

Ligands and Environments

2 bound ligands:
Ligand GLU 8 x GLU
Ligand PLP 4 x PLP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL18U1
Spacegroup: P21
Unit cell:
a: 68.39Å b: 164.544Å c: 109.24Å
α: 90° β: 105.81° γ: 90°
R-values:
R R work R free
0.169 0.167 0.227
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

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Citations

1 review citation

Implications of hydrogen sulfide in colorectal cancer: Mechanistic insights and diagnostic and therapeutic strategies.
Lin et al. (2023)
3 other articles