› 6jdr

X-ray diffraction
2.5Å resolution

Crystal structure of methylated PRRSV nsp10 (helicase)

Released:
DOI: 10.2210/pdb6jdr/pdb
PDB entry 6jdr coloured by chain, front view.
PDB entry 6jdr coloured by chain, side view.
PDB entry 6jdr coloured by chain, top view.
Source organism: Porcine reproductive and respiratory syndrome virus
Primary publication:
Helicase of Type 2 Porcine Reproductive and Respiratory Syndrome Virus Strain HV Reveals a Unique Structure.
Tang C, Deng Z, Li X, Yang M, Tian Z, Chen Z, Wang G, Wu W, Feng WH, Zhang G, Chen Z
Viruses 12 (2020)
PMID: 32075207

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-125447 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
PP1b Chain: A
Molecule details ›
Chain: A
Length: 441 amino acids
Theoretical weight: 49.03 KDa
Source organism: Porcine reproductive and respiratory syndrome virus
Expression system: Escherichia coli
UniProt:
  • Canonical: J9XNG9 (Residues: 644-1084; Coverage: 30%)
Sequence domains: Viral (Superfamily 1) RNA helicase

Ligands and Environments

2 bound ligands:
Ligand ZN 5 x ZN
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U1
Spacegroup: P43212
Unit cell:
a: 93.158Å b: 93.158Å c: 148.108Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.237 0.235 0.272
Expression system: Escherichia coli

Quick links

Citations

8 review citations

Insights into SARS-CoV-2 genome, structure, evolution, pathogenesis and therapies: Structural genomics approach.
Naqvi et al. (2020)
7 more

1 mention without citation

Helicase of Type 2 Porcine Reproductive and Respiratory Syndrome Virus Strain HV Reveals a Unique Structure.
Tang et al. (2020)