6j95

X-ray diffraction
2Å resolution

Crystal structure of CYP97A3 in complex with retinal

Released:
Source organism: Arabidopsis thaliana
Primary publication:
Structural basis for plant lutein biosynthesis from α-carotene.
Proc. Natl. Acad. Sci. U.S.A. (2020)
PMID: 32513704

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein LUTEIN DEFICIENT 5, chloroplastic Chain: A
Molecule details ›
Chain: A
Length: 519 amino acids
Theoretical weight: 58.46 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli
UniProt:
  • Canonical: Q93VK5 (Residues: 78-595; Coverage: 87%)
Gene names: At1g31800, CYP97A3, F5M6.19, LUT5
Sequence domains: Cytochrome P450

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P212121
Unit cell:
a: 58.481Å b: 82.097Å c: 110.339Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.167 0.165 0.197
Expression system: Escherichia coli