PDBe 6j8f

X-ray diffraction
2.28Å resolution

Crystal structure of SVBP-VASH1 with peptide mimic the C-terminal of alpha-tubulin


Function and Biology Details

Reaction catalysed:
Cleavage of the -Glu-|-Tyr bond to release the C-terminal tyrosine residue from the native tyrosinated tubulin. Inactive on Z-Glu-Tyr.
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Small vasohibin-binding protein Chain: A
Molecule details ›
Chain: A
Length: 46 amino acids
Theoretical weight: 5.41 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
  • Canonical: Q8N300 (Residues: 3-48; Coverage: 70%)
Gene names: CCDC23, SVBP
Tubulinyl-Tyr carboxypeptidase 1 Chain: B
Molecule details ›
Chain: B
Length: 238 amino acids
Theoretical weight: 27.67 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
  • Canonical: Q7L8A9 (Residues: 69-306; Coverage: 65%)
Gene names: KIAA1036, VASH, VASH1
8-mer peptide Chain: C
Molecule details ›
Chain: C
Length: 8 amino acids
Theoretical weight: 915 Da
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U1
Spacegroup: P21212
Unit cell:
a: 66.95Å b: 108.377Å c: 46.896Å
α: 90° β: 90° γ: 90°
R R work R free
0.202 0.2 0.237
Expression systems:
  • Escherichia coli 'BL21-Gold(DE3)pLysS AG'
  • Escherichia coli
  • Not provided