› 6iw8

X-ray diffraction
2.8Å resolution

Crystal structure of Importin-alpha and wild-type GM130

Released:
DOI: 10.2210/pdb6iw8/pdb
PDB entry 6iw8 coloured by chain, front view.
PDB entry 6iw8 coloured by chain, side view.
PDB entry 6iw8 coloured by chain, top view.
Source organisms:
Primary publication:
Ran pathway-independent regulation of mitotic Golgi disassembly by Importin-α.
Chang CC, Chen CJ, Grauffel C, Pien YC, Lim C, Tsai SY, Hsia KC
Nat Commun 10 4307 (2019)
PMID: 31541088

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156518 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Golgin subfamily A member 2 Chain: A
Molecule details ›
Chain: A
Length: 53 amino acids
Theoretical weight: 5.77 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q08379 (Residues: 13-60; Coverage: 5%)
Gene name: GOLGA2
Importin subunit alpha-1 Chain: C
Molecule details ›
Chain: C
Length: 433 amino acids
Theoretical weight: 46.9 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P52293 (Residues: 70-498; Coverage: 81%)
Gene names: Kpna2, Rch1
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE TPS 05A
Spacegroup: P212121
Unit cell:
a: 75.399Å b: 78.628Å c: 90.318Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.227 0.227 0.255
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Karyopherin-mediated nucleocytoplasmic transport.
Wing et al. (2022)
6 more

1 mention without citation

Microtubule binding of the human augmin complex is directly controlled by importins and Ran-GTP.
Ustinova et al. (2023)