› 6iw6

X-ray diffraction
2.4Å resolution

Crystal structure of the Lin28-interacting module of human TUT4

Released:
DOI: 10.2210/pdb6iw6/pdb
PDB entry 6iw6 coloured by chain, front view.
PDB entry 6iw6 coloured by chain, side view.
PDB entry 6iw6 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of the Lin28-interacting module of human terminal uridylyltransferase that regulates let-7 expression.
Yamashita S, Nagaike T, Tomita K
Nat Commun 10 1960 (2019)
PMID: 31036859

Function and Biology Details

Reaction catalysed: 
UTP + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178318 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Terminal uridylyltransferase 4 Chains: A, B
Molecule details ›
Chains: A, B
Length: 448 amino acids
Theoretical weight: 51.76 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5TAX3 (Residues: 253-723; Coverage: 27%)
Gene names: KIAA0191, TUT4, ZCCHC11
Sequence domains:

Ligands and Environments

3 bound ligands:
Ligand ZN 4 x ZN
Ligand FLC 2 x FLC
Ligand EDO 8 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-17A
Spacegroup: I212121
Unit cell:
a: 113.27Å b: 127.83Å c: 168.8Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.211 0.21 0.237
Expression system: Escherichia coli

Quick links

Citations

4 review citations

A tale of non-canonical tails: gene regulation by post-transcriptional RNA tailing.
Yu et al. (2020)
3 more