PDBe 6iv8

X-ray diffraction
2.15Å resolution

the selenomethionine(SeMet)-derived Cas13d binary complex

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct RNA molecules
Macromolecules (3 distinct):
The selenomethionine (SeMet)-labeled Cas13d Chains: A, C
Molecule details ›
Chains: A, C
Length: 930 amino acids
Theoretical weight: 108.76 KDa
Source organism: uncultured Ruminococcus sp.
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A1C5SD84 (Residues: 1-922; Coverage: 100%)
Gene name: SAMEA3545300_02264
RNA (51-MER) Chain: B
Molecule details ›
Chain: B
Length: 51 nucleotides
Theoretical weight: 16.24 KDa
Source organism: uncultured Ruminococcus sp.
Expression system: Escherichia coli
RNA (53-MER) Chain: D
Molecule details ›
Chain: D
Length: 53 nucleotides
Theoretical weight: 16.88 KDa
Source organism: uncultured Ruminococcus sp.
Expression system: Escherichia coli

Ligands and Environments

1 bound ligand:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U1
Spacegroup: P212121
Unit cell:
a: 65.164Å b: 145.733Å c: 249.383Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.19 0.189 0.21
Expression system: Escherichia coli