6irc

X-ray diffraction
3.54Å resolution

C-terminal domain of Drosophila phospholipase b NORPA, methylated

Released:

Function and Biology Details

Reaction catalysed:
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H(2)O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
PDBe Complex ID:
PDB-CPX-146510 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase Chain: A
Molecule details ›
Chain: A
Length: 233 amino acids
Theoretical weight: 27.53 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P13217 (Residues: 863-1095; Coverage: 21%)
Gene names: CG3620, PLC-beta, norpA
Sequence domains: Protein of unknown function (DUF1154)

Ligands and Environments

No bound ligands
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P6122
Unit cell:
a: 119.209Å b: 119.209Å c: 116.059Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.247 0.244 0.296
Expression system: Escherichia coli BL21(DE3)